12234026安捷伦Sps 24 Coll. Rack/F

POLY-L-LYSINE COATED SLIDES

approximately 35 minutes and the slides pass through the following solutions in this order. i) 5% chromic acid x1 ii) Distilled water x5 iii) P-L-L solution x1 7. Dry the slides in the rack in the 37°C oven for 24 hours.

Cell Cultures

, set up a second series of dilutions, this time diluting by 1/2 each time. That is, transfer 5.0 ml of the 10 culture to 5.0 ml of fresh broth and mix thoroughly. Use 5.0 ml of this and transfer to 5.0 ml of fresh broth to produce a 1/4 dilution. Repeat

miRNA microarrays（McManus Lab）(芯片）

Ambion 3x hybridization buffer (Cat # 1567 mirVana™ miRNA Bioarray Essentials Kit) 22x25 mm lifter sliPS (Erie 22x25I-M-5226) a) Heat 3x hyb buffer at 65℃ and vortex to resuspend b) Add 5 to 10 100 μL drops of 5x SSC