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文献和实验capacity and a loading volume of up to 800 µl. Fused-silica capillary, 100 µm I.D. x 365 µm O.D. (Agilent Technologies or Polymicro Technologies) Fused-silica capillary (size and length depend on the desired split flow rate, see Step 10) Gold
by PAGE usingthe following formula: Indel percentage (%) = (1 . √(1 . x )) × 100, where x = ( a + b )/( a + b + c ) In this formula, the number “ a ” and “ b ” represent therelative quantities of the cleaved bands, while “ c ” equals
sodium acetate solution to pH 6.0 with the 3M acetic acid solution. Filter sterilize using a 0.2 micron filter. Store at room temperature. Ethanol/acetate mix Ethanol (100%) 950 ml Sodium acetate pH=6.0 50 ml 1000 ml 3X SSC
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