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文献和实验Organotypic raft culture of primary human keratinocytes (PHKs)
seem to leave them for 8-14 days to grow/differentiate Add in BrdU 8-12 hours before harvesting (see separate BrdU protocol) Suppliers Stainless steel wire mesh 3 1 /16 inch 40 mesh .010 (=wire size) 304 SS circle, item #105493
Mag-Binds Plant DNA Kit Magnetic 96 Protocol
isolation protocol. Mechanical tissue disruption: Place sample into a stainless steel grinding jar with appropriate steel beads. Freeze samples in the stainless steel grinding jar using liquid nitrogen for 1 minute. Immediately attach
buffer, pH6.0 unless otherwise indicated overleaf) until boiling in a stainless steel pressure cooker. Cover but do not lock lid. 5. Position slides into metal staining racks (do not place slides close together as uneven staining may occur) and lower
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