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文献和实验Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions
) Tabletop ultracentrifuge (Beckman Coulter, model TL‐100) Nanodrop spectrophotometer (Thermo Fisher Scientific; also see unit 3.10
Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
Iscove's modified Dulbecco's medium (IMDM; Invitrogen, cat. no. 12440‐046) HCFC expansion medium: X‐Vivo 15 medium (Lonza, cat. no. 04‐744Q) supplemented with 10 ng/ml Flt
High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
‐, 20‐, 100‐, 1000‐, 5000‐, and 25000‐fold. The fluorescence intensity of 3‐nitrotyrosine from each diluted assay was plotted with folds of dilution ( X ‐dimension label). The different capture antibodies are color coded in the inset key. The results
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