- ￥2108.68
- Thermo Fisher
- 365I2331
- 2025年08月25日
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文献和实验Evaluating Modulators of “Regulator of G‐protein Signaling” (RGS) Proteins
7.5), 1 mM EDTA, 10 mM MgCl 2 , 10 µM GTP, 10 µg/ml GDP antibody, and 2 nM fluorescent nucleotide tracer; for the protocol below, this will be prepared as a 1.1× solution (18 µl added to a final 20 µl
Materials: 250 µL of fresh mouse blood in plastic tubes containing EDTA. RBC lysis buffer (388 mM NH4 Cl, 29.7 mM NaHCO3 , 25 µM Na2 EDTA) 20.75g. NH4 CL 2.5g NaHCO3 0.093g Na2 EDTA 1L H2
Southern Hybridization Protocols
solution is the same. 60 ℃ for lower stringency Southern hybridization. 65℃ for higher stringency Southern hybridization. For probe labelling: Use RadPrime DNA Labeling System (Invitrogen) Denature 25 ng (1 µ l) in 19 µ l of sterile
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