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文献和实验Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
containing 15-20 drops (20 µL per drop) of 7.5 µg/mL cytochalasin B, and one glass-bottom dish containing 3-4 drops (4 µL per drop) of HEPES containing 5% SPS. Prewarm the dishes at 37°C on a thermo plate. 4. Attach the holding
(Fisher M/4056/17)、Acetonitrile(Fisher A/0626/17)、Trypsin (Promega V528)、Trypsin resolve solution(Promega V530)、Ammonium bicarbonate(Sigma A6141)、ZipTip μC-18(Millipore ZTC18M096)、TFA(GE HealthCare)、0.2ml and 0.5ml EP tube(eppendorf)、50ml EP 0.5ml EP
(Fisher Scientific) 1.5‐ml screw‐cap polypropylene centrifuge tubes 55°C and 65°C heating
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