26082-5040赛默飞TG-POLAR 30m x 0.

Clonality - X Chromosome Inactivation Assay

(1:1:1, v:v:v) (Gibco) 3 M Na Acetate, pH 5.2 (Life Technologies) Isopropanol Glycogen, 10 mg/ml (GenHunter) 70% ethanol Buffer 1 (50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 50 mM NaCl) Hha I restriction enzyme (Life Technologies) REact 2 Buffer

x2检验

　　T1.1 =26.2 　　T1.2 =43-26.2=16.8 　　T2.1 =53-26.2=26.8 　　T2.2 =44-26.2=17.2 　　3.计算x2 值 按公式20.12代入 　　4．查x2 值表求P值 　　在查表之前应知本题自由度。按x2 检验的自由度v=（行数-1）（列数-1），则该题的自由度v=（2-1）（2-1）=1，查x2 界值表（附表20-1），找到x2 0.001

Karyotyping of X. tropicalis

  We have been karyotyping animals in the Grainger lab to confirm that the stocks maintained in the lab are indeed X. tropicalis and to establish that wild-caught animals being used for gynogenesis are also X. tropicalis . We recently