60180-830赛默飞Super Clean 三组分过滤器

Temperature-Induced Phase Partitioning for Protein Purification

. Traditionally, the removal of polymer from protein in an aqueous two-phase separation requires either back extraction into a saltrich phase, ultrafiltration, diafiltration, or a chromatographic step, e.g., ion exchange (see Chapter 32). Thermo separating

DNA Sequencing

flask. Add acrylamide, TBE and water (total volume 135 mL). Place in 37℃ waterbath to dissolve urea (do not overheat!). Clean the glass plates, spacers, comb of the gel apparatus - first with water, then with 70% Ethanol. Siliconize bottom plate

A/B/C Random Amplification Protocol

DNA. Round B PCR   30 µL  Round A DNA product   20 µL 10X Thermo Pol PCR Buffer     2 µL 25 mM dNTP     4 µL  100 µM Primer B Cy5 (sample) or Cy3 (reference DNA)     2 µL  Ampli Taq  0.1 µL Pfu Turbo 142 µL dH 2 O