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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
"-20°C/-80°C"
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
- 英文名:
Customized Caenorhabditis elegans dnj-21 Protein (in vitro E.coli)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20ug
Alternative Name(s)
Mitochondrial import inner membrane translocase subunit TIM14 Alternative name(s): DnaJ homolog subfamily C member 21Editorial/Sponsord
EditorialUniprot ID
P91454Gene Names
dnj-21Organism
Caenorhabditis elegansAASequence
MTGGLIVAGLGLAAVGFGARYVLRNQALIKKGMEAIPVAGGAFSNYYRGGFDQKMSRAEAAKILGVAPSAKPAKIKEAHKKVMIVNHPDRGGSPYLAAKINEAKDLMESSKSExpression Region
1-112aaSequence Info
Full lengthSource
in vitro E.coliSource Notice
Mammalian cell expression systems and other species are available. Please inquire.Tag Info
InquireMW
InquireList Price
1396Purity
Greater than 85% as determined by SDS-PAGE.Storage Buffer
Tris/PBS-based buffer, 6% TrehaloseStorage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.Endotoxin
Not Test. Endotoxin removal service is available for free upon you request.产品类型
Transmembrane-Protein备注
**产品信息可能有变动,请以官网信息为准
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文献和实验**产品信息可能有变动,请以官网信息为准
Reconstitution of Mitochondrial Presequence Translocase into Proteoliposomes
of the mitochondrial presequence translocase (TIM23 complex). This highly versatile and dynamic protein complex is the main protein translocation machinery of the inner mitochondrial membrane and mediates the import of precursor proteins with N-terminal presequences
线粒体荧光探针大全:TMRM,Mitotracker,JC-1
exceptions, although their membrane potential�independent uptake and fluorescence has been questioned in some cell types.11,12 Mitochondrion-selective reagents enable researchers to probe mitochondrial activity, localization and abundance,13,14
严格的情况下可以用次氯酸钠溶液的(14% m/V 的贮存液进行 20 倍稀释)表面灭菌 5 min。所有材料在使用前需冷却到 2~4°C。 2.2 匀浆缓冲液 为保证产率,在收集并冷却好植物组织后,应该立即匀浆开始线粒体的提取。基本的匀浆缓冲液包括 0.3~0.4 mol/L 的渗透物质(蔗糖或甘露醇) 、2~5 mmol/L 的四价阳离子螯合剂(EDTA 或 EGTA ) 、20~50 mmol/L 碱 性缓冲系统(MOPS、TES 或焦磷酸钠)、5~20 mmol/L 还原剂(半胱
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