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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
"-20°C/-80°C"
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
- 英文名:
Customized Komagataella phaffii LCL3 Protein (in vitro E.coli)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20ug
Alternative Name(s)
Probable endonuclease LCL3 EC= 3.1.-.-Editorial/Sponsord
EditorialUniprot ID
C4QW04Gene Names
LCL3Organism
Komagataella phaffiiAASequence
MAQSNQHVSIYNPKVIVYSIGLTTAILASMSIYRSHFVRFSTSLDVPKTLFRTKHLHGKV TSVGDGDNFHFYHLPGGIFAGWGWIRETPEINKFRKLKNKTIHVRLCGVDAPERSHFGKP SQPYSEEALQWLRQFILGKKVKVKPLSVDQYNRIVGRVFIFRWNGWNDVSEEMLRNGVAI VYENKSSAEFDGMKERYLKVENKAKKKKKGLWGIERGLTPGEYKRLYKExpression Region
1-228aaSequence Info
Full lengthSource
in vitro E.coliSource Notice
Mammalian cell expression systems and other species are available. Please inquire.Tag Info
InquireMW
InquireList Price
1544Purity
Greater than 85% as determined by SDS-PAGE.Storage Buffer
Tris/PBS-based buffer, 6% TrehaloseStorage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.Endotoxin
Not Test. Endotoxin removal service is available for free upon you request.产品类型
Transmembrane-Protein备注
**产品信息可能有变动,请以官网信息为准
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文献和实验**产品信息可能有变动,请以官网信息为准
Partial Endonuclease Digestion
, tubes 2 through 4 contain 20 µl, and tube 5 contains 10 µl (see diagram below). Place the tubes on ice. Add restriction endonuclease (3-10 units of enzyme per µg of DNA) to tube 1, mix quickly, and place the tube on ice. Add 10 µl
Restriction Endonuclease Digestion of DNA
The ability to cleave DNA at specific sites is one of the cornerstones of today’s methods of DNA manipulation. Restriction endonucleases are bacterial enzymes that cleave duplex DNA at specific target sequences with the production of defined
在核酸水解酶中,为可水解分子链内部磷酸二酯键生成寡核苷酸的酶,与核酸外切酶相对应。从对底物的特异性来看,可分为 DNaseⅠ、 DNaseⅡ等仅分解 DNA的酶;脾脏 RNase、 RNaseT1 等仅分解 RNA的酶。如链孢霉( Neurospora)的核酸酶就是既分解 DNA又分解 RNA的酶。一般来说,大都不具碱基特异性,但也有诸如脾脏 RNase、 RNaseT1 等或限制性内切酶那种能够识别并切断特定的碱基或碱基序列的酶。
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