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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
"-20°C/-80°C"
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
- 英文名:
Customized Danio rerio rnaseka Protein (in vitro E.coli)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20ug
Alternative Name(s)
Ribonuclease kappa-A; RNase K-A; RNase kappa-A EC= 3.1.-.-Editorial/Sponsord
EditorialUniprot ID
Q0P467Gene Names
rnasekaOrganism
Danio rerioAASequence
MVSLLFCGPKLAACGLVLSIWGVIMLALLGIFFTTHSAILIEDVPLTEEDLHSQDTPPQS VYKLYNQVGYNCFIAAVIYVGIGFLSFCQVRLNKRKEYLVHExpression Region
1-101aaSequence Info
Full lengthSource
in vitro E.coliSource Notice
Mammalian cell expression systems and other species are available. Please inquire.Tag Info
InquireMW
InquireList Price
1382Purity
Greater than 85% as determined by SDS-PAGE.Storage Buffer
Tris/PBS-based buffer, 6% TrehaloseStorage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.Endotoxin
Not Test. Endotoxin removal service is available for free upon you request.产品类型
Transmembrane-Protein备注
**产品信息可能有变动,请以官网信息为准
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文献和实验**产品信息可能有变动,请以官网信息为准
亦称k粒。它存在于放毒型草履虫的细胞质中,能进行自体复制,显有与病毒相似的性质,一般认为是寄生性病毒。卡巴粒的直径为 0.2— 0.5微米,长度为 0.5— 5微米。在具有放毒作用强的放毒型中,一个细胞内有 400— 1600个卡巴粒,这种粒子也可向草履虫的培养液中释放,可杀死摄取这种粒子的敏感型草履虫。与卡巴粒相类似的粒子有λ、σ、γ、δ等粒子。
RNA Extraction by the Proteinase K Method
This method of RNA extraction relies on a relatively gentle lysis procedure that should burst the cells, but leave the nuclei intact. Contamination of a relatively RNase-free cytoplasmic environment with nuclear nucleases is thus minimised
RNase Protection Assay (Bowtell Lab)
1. Cut 10ug of plasmid DNA where you want the transcript to end. 2. Add an equal volume of 2X PK buffer: 200mM TrisHCl pH 7.5 25mM EDTA 300mM NaCl 2% SDS 200ug/ml proteinase K 3. 37� for 30min and then extract with phenol
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