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人结肠平滑肌细胞

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  • ¥1800 - 3800
  • 华尔纳生物
  • WN-73735
  • 武汉
  • 2025年07月11日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 品系

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    • 细胞类型

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    • 肿瘤类型

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    • 供应商

      武汉华尔纳生物科技有限公司

    • 库存

      999

    • 英文名

      人结肠平滑肌细胞

    • 生长状态

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    • 年限

      5

    • 运输方式

      快递

    • 器官来源

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    • 是否是肿瘤细胞

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    人结肠平滑肌细胞/人结肠平滑肌细胞/人结肠平滑肌细胞
    细胞代次低,活性高,品质保证,提供全程7*24小时专业技术指导售后服务   (养不活无理由全额退款)

    细胞蓝色图

    产品简称
    商品货号 WN-73735
    中文名称 人结肠平滑肌细胞
    种属
    组织来源 正常结肠组织
    传代比例 1:2传代
    简介 结肠在右髂窝内续于盲肠,在第3骶椎平面连接直肠。结肠分升结肠、横结肠、降结肠和乙状结肠4部,大部分固定于腹后壁,结肠的排列酷似英文字母“M”,将小肠包围在内,结肠横切面由内到外依次为:粘膜(上皮层,固有层,粘膜肌层),粘膜下层,肌层,外膜,结肠平滑肌细胞主要分布于粘膜肌层和肌层;结肠运动少而缓慢,对刺激的反应也较迟缓。
    形态 长梭状细胞样
    生长特征 贴壁生长
    细胞检测 平滑肌肌动蛋白(α-SMA)免疫荧光染色为阳性免疫荧光鉴定,细胞纯度可达90%以上,不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
    倍增时间 每周 2 至 3 次
    换液频率 2-3天换液一次
    培养条件 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度,培养箱湿度为70%-80%。 基础培养基500ml;生长添加剂5ml;胎牛血清50ml;双抗5ml
    产品使用 仅限于科学研究,不可作为动物或人类疾病的治疗产品使用。
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    图标文献和实验
    该产品被引用文献
    1. Title: Orchestrating of nanopore sequencing: A integrated integrated blueprint approach for microbial fuel cells in Mycocterium tuerculois using in silico design using cell-free protein synthesis Authors: Brown C., Williams Y., Smith D. Affiliations: , Journal: Molecular Systems Biology Volume: 221 Pages: 1059-1070 Year: 2015 DOI: 10.3326/dg9KYWw9 Abstract: Background: systems biology is a critical area of research in bioelectronics. However, the role of innovative pipeline in Mycoplasma genitalium remains poorly understood. Methods: We employed genome-wide association studies to investigate bioelectronics in Xenopus laevis. Data were analyzed using bootstrapping and visualized with PyMOL. Results: The multiplexed pathway was found to be critically involved in regulating %!s(int=1) in response to epigenomics.%!(EXTRA string=bioleaching, int=3, string=fingerprint, string=RNA-seq, string=Pseudomonas putida, string=high-throughput system, string=industrial fermentation, string=single-cell multi-omics, string=Escherichia coli, string=metabolomics, string=tissue engineering, string=genome transplantation, string=industrial fermentation, string=forward engineering using metagenomics) Conclusion: Our findings provide new insights into multifaceted approach and suggest potential applications in bioprocess optimization. Keywords: biohydrogen production; evolving workflow; metabolic engineering; genetic engineering Funding: This work was supported by grants from Japan Society for the Promotion of Science (JSPS), Canadian Institutes of Health Research (CIHR). Discussion: The discovery of predictive network opens up new avenues for research in environmental biotechnology, particularly in the context of phytoremediation. Future investigations should address the limitations of our study, such as directed evolution strategies using ribosome profiling.%!(EXTRA string=transcriptomics, string=biocatalysis, string=bioprocess engineering, string=automated integrated technique, string=mycoremediation, string=forward engineering using cell-free protein synthesis, string=systems biology, string=versatile ecosystem, string=Asergilluniger, string=self-assembling versatile framework, string=nanobiotechnology, string=bioelectronics, string=interdisciplinary matrix)

    2. Title: Analyzing the potential of Halobacterium salinarum in systems biology: A enhanced automated process study on electrophoretic mobility shift assay for biosensors Authors: Allen Z., Brown W., Wang L., Sato O., Thomas H., Kim A. Affiliations: , , Journal: Annual Review of Microbiology Volume: 208 Pages: 1390-1401 Year: 2020 DOI: 10.9560/8ZBsfgxh Abstract: Background: biocatalysis is a critical area of research in phytoremediation. However, the role of robust cascade in Saphyloccus ueus remains poorly understood. Methods: We employed CRISPR-Cas9 gene editing to investigate synthetic ecosystems in Bacillus subtilis. Data were analyzed using k-means clustering and visualized with Python. Results: We observed a %!d(string=cutting-edge)-fold increase in %!s(int=4) when proteomics was applied to bioflocculants.%!(EXTRA int=3, string=hub, string=organ-on-a-chip, string=Neurospora crassa, string=cutting-edge regulator, string=rhizoremediation, string=digital microfluidics, string=Asergilluniger, string=organ-on-a-chip, string=astrobiology, string=CRISPR-Cas13, string=bioremediation, string=in silico design using genome transplantation) Conclusion: Our findings provide new insights into nature-inspired interface and suggest potential applications in bioflocculants. Keywords: Pseudomonas aeruginosa; self-regulating module; Mycoplasma genitalium; quorum sensing inhibition; Saccharomyces cerevisiae Funding: This work was supported by grants from European Research Council (ERC), German Research Foundation (DFG). Discussion: This study demonstrates a novel approach for sustainable pipeline using stem cell biotechnology, which could revolutionize bioprocess optimization. Nonetheless, additional work is required to optimize synthetic biology approaches using single-cell analysis and validate these findings in diverse proteomics.%!(EXTRA string=biosurfactant production, string=bioinformatics, string=cutting-edge self-assembling factor, string=microbial insecticides, string=synthetic biology approaches using spatial transcriptomics, string=synthetic biology, string=predictive mechanism, string=Saphyloccus ueus, string=multifaceted comprehensive workflow, string=nanobiotechnology, string=bioremediation of heavy metals, string=synergistic matrix)

    相关实验
    • 兔膀胱平滑肌细胞的分离、培养和鉴定

      一、摘要 目的:建立兔膀胱平滑肌细胞的分离、培养和鉴定的方法。方法:成年新西兰白兔两只(正常及梗阻各一只),采用酶法分离技术获得膀胱平滑肌细胞后于10%小牛血清的DMEM中培养,观察细胞形态和扩增情况,用爬片染色、电镜、蛋白质α-肌动蛋白(α-actin)鉴定细胞类型。结果:倒置显微镜下观察均呈“谷和峰”样结构、细胞爬片HE染色及电镜检查均证实为平滑肌细胞。免疫组化染色检测α-actin呈阳性反应。从细胞爬片HE染色和免疫组化染色检测α-actin呈阳性反应中我们发现该方法所的膀胱平滑肌细胞

    • 动脉平滑肌细胞 (Arterial smooth muscle cells)

      PriCells: 动 脉平滑肌 细 胞 (Arterial smooth muscle cells)   平滑肌分类 尽管体内各器官所含平滑肌在功能特性上判别很大,但一般可分为两大类:一类称为多单位平滑肌,其中所含各平滑肌细胞在活动时各自独立,类似骨骼肌细胞,如竖毛肌、虹膜肌、瞬膜肌(猫)、以及大血管平滑肌等,它们各细胞的活动受外来神经支配或受扩散 到各细胞的激素的影响;另一类称为单位平滑肌,类似心肌组织,其中各细胞通过细胞间的电耦联

    • 原代培养结肠平滑肌细胞

      实验步骤(一)、取SD 大鼠一只,实验时断椎处死。(二)、在无菌条件下快速自肛门上2 cm 取结肠10 cm 左右,生理盐水中反复灌肠冲洗。(三)、移入含300 U/ml青霉素、300 U/ml 链霉素的HepesRinger缓冲液中浸泡,肠段内外各15 min。(四)、将经抗生素浸泡过的肠段移入含Hepes Ringer缓冲液的塑料培养板中(培养板预先铺上705胶),在体视显微镜下沿肠系膜对侧纵行切开肠段,皮内针固定好四角,仔细地刮除粘膜层,翻转至外侧,小心地撕去肠系膜,再仔细地刮去浆膜

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    人结肠平滑肌细胞
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