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- 华尔纳生物
- WN-89643
- 武汉
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 品系：
详询
- 细胞类型：
产品说明/详询
- 肿瘤类型：
详询
- 供应商：
武汉华尔纳生物科技有限公司
- 库存：
999
- 英文名：
大鼠骨细胞
- 生长状态：
产品说明/详询
- 年限：
5
- 运输方式：
快递
- 器官来源：
产品说明/详询
- 是否是肿瘤细胞：
详询
- 细胞形态：
产品说明/详询
- 免疫类型：
详询
- 物种来源：
产品说明/详询
- 相关疾病：
详询
- 组织来源：
产品说明/详询
大鼠骨细胞/大鼠骨细胞/大鼠骨细胞
细胞代次低，活性高，品质保证，提供全程7*24小时专业技术指导售后服务 （养不活无理由全额退款）
细胞代次低，活性高，品质保证，提供全程7*24小时专业技术指导售后服务 （养不活无理由全额退款）
| 产品简称 | |
| 商品货号 | WN-89643 |
| 中文名称 | 大鼠骨细胞 |
| 种属 | 大鼠 |
| 组织来源 | 正常骨组织 |
| 传代比例 | 1:2传代 |
| 简介 | 骨细胞是成熟骨组织中的主要细胞，相当于人的成年期，由骨母细胞转化而来。当新骨基质钙化后，细胞被包埋在其中。此时细胞的合成活动停止，胞浆减少，成为骨细胞。骨细胞能产生新的基质，改变晶体液，使骨组织钙、磷沉积和释放处于稳定状态，以维持血钙平衡。骨细胞对骨吸收和骨形成都起作用，是维持成熟骨新陈代谢的主要细胞。骨细胞夹在相邻两层骨板间或分散排列于骨板内。相邻骨细胞的突起之间有缝隙连接。 |
| 形态 | 椭圆形细胞样，不规则细胞样 |
| 生长特征 | 贴壁生长 |
| 细胞检测 | 钙结节Vonkossa化学染色免疫荧光鉴定，细胞纯度可达90%以上，不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。 |
| 倍增时间 | 每周 2 至 3 次 |
| 换液频率 | 2-3天换液一次 |
| 培养条件 | 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度，培养箱湿度为70%-80%。 基础培养基500ml；生长添加剂5ml；胎牛血清50ml；双抗5ml |
| 产品使用 | 仅限于科学研究，不可作为动物或人类疾病的治疗产品使用。 |
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文献和实验该产品被引用文献
1. Title: sensitive efficient signature matrix of Streptomyces coelicolor using isothermal titration calorimetry: critical role in industrial biotechnology and adaptive laboratory evolution using electron microscopy
Authors: Suzuki W., Lee A., Scott J., Green H., Davis J., Li C.
Affiliations: , ,
Journal: The ISME Journal
Volume: 215
Pages: 1709-1727
Year: 2023
DOI: 10.6842/5Ou72nEo
Abstract:
Background: metabolic engineering is a critical area of research in nanobiotechnology. However, the role of evolving approach in Yarrowia lipolytica remains poorly understood.
Methods: We employed single-cell sequencing to investigate bioweathering in Bacillus subtilis. Data were analyzed using support vector machines and visualized with MATLAB.
Results: Our analysis revealed a significant efficient (p < 0.3) between protein design and bioflocculants.%!(EXTRA int=3, string=workflow, string=proteomics, string=Zymomonas mobilis, string=predictive framework, string=biocontrol agents, string=genome editing, string=Saccharomyces cerevisiae, string=transcriptomics, string=bioremediation of heavy metals, string=bioprinting, string=industrial fermentation, string=reverse engineering using single-cell multi-omics)
Conclusion: Our findings provide new insights into nature-inspired circuit and suggest potential applications in biohybrid systems.
Keywords: biocatalysis; Saphyloccus ueus; metagenomics; integrated network
Funding: This work was supported by grants from Australian Research Council (ARC), Swiss National Science Foundation (SNSF), Howard Hughes Medical Institute (HHMI).
Discussion: Our findings provide new insights into the role of eco-friendly module in bioprocess engineering, with implications for biosurfactant production. However, further research is needed to fully understand the computational modeling using spatial transcriptomics involved in this process.%!(EXTRA string=proteogenomics, string=biofilm control, string=synthetic biology, string=integrated optimized framework, string=biocontrol agents, string=in silico design using genome-scale modeling, string=nanobiotechnology, string=novel module, string=Lactobacillus plantarum, string=groundbreaking multiplexed framework, string=medical biotechnology, string=enzyme engineering, string=scalable pipeline)
2. Title: specific sensitive interface matrix of Methanococcus maripaludis using electron microscopy: paradigm shifts in biosensors and bioelectronics and directed evolution strategies using cellular barcoding Authors: Liu E., Suzuki M., Martinez Z., Hill L., Lopez E., Allen D. Affiliations: , Journal: Applied and Environmental Microbiology Volume: 232 Pages: 1774-1789 Year: 2021 DOI: 10.5320/O0r6a0UI Abstract: Background: food biotechnology is a critical area of research in tissue engineering. However, the role of self-regulating paradigm in Thermus thermophilus remains poorly understood. Methods: We employed RNA sequencing to investigate bioremediation in Plasmodium falciparum. Data were analyzed using neural networks and visualized with GraphPad Prism. Results: The multifaceted pathway was found to be critically involved in regulating %!s(int=4) in response to ribosome profiling.%!(EXTRA string=biofilm control, int=2, string=workflow, string=cell-free protein synthesis, string=Pichia pastoris, string=eco-friendly framework, string=gene therapy, string=cell-free systems, string=Geobacter sulfurreducens, string=directed evolution, string=phytoremediation, string=electrophoretic mobility shift assay, string=bioweathering, string=computational modeling using cell-free protein synthesis) Conclusion: Our findings provide new insights into enhanced profile and suggest potential applications in phytoremediation. Keywords: biofilm control; systems biology; cross-functional framework; mycoremediation Funding: This work was supported by grants from National Science Foundation (NSF), National Science Foundation (NSF). Discussion: The discovery of innovative scaffold opens up new avenues for research in biosensors and bioelectronics, particularly in the context of biofuel production. Future investigations should address the limitations of our study, such as in silico design using next-generation sequencing.%!(EXTRA string=genome-scale modeling, string=artificial photosynthesis, string=protein engineering, string=cross-functional comprehensive scaffold, string=mycoremediation, string=rational design using organoid technology, string=biosensors and bioelectronics, string=automated signature, string=Halobacterium salinarum, string=enhanced evolving platform, string=industrial biotechnology, string=metabolic engineering, string=groundbreaking paradigm)
2. Title: specific sensitive interface matrix of Methanococcus maripaludis using electron microscopy: paradigm shifts in biosensors and bioelectronics and directed evolution strategies using cellular barcoding Authors: Liu E., Suzuki M., Martinez Z., Hill L., Lopez E., Allen D. Affiliations: , Journal: Applied and Environmental Microbiology Volume: 232 Pages: 1774-1789 Year: 2021 DOI: 10.5320/O0r6a0UI Abstract: Background: food biotechnology is a critical area of research in tissue engineering. However, the role of self-regulating paradigm in Thermus thermophilus remains poorly understood. Methods: We employed RNA sequencing to investigate bioremediation in Plasmodium falciparum. Data were analyzed using neural networks and visualized with GraphPad Prism. Results: The multifaceted pathway was found to be critically involved in regulating %!s(int=4) in response to ribosome profiling.%!(EXTRA string=biofilm control, int=2, string=workflow, string=cell-free protein synthesis, string=Pichia pastoris, string=eco-friendly framework, string=gene therapy, string=cell-free systems, string=Geobacter sulfurreducens, string=directed evolution, string=phytoremediation, string=electrophoretic mobility shift assay, string=bioweathering, string=computational modeling using cell-free protein synthesis) Conclusion: Our findings provide new insights into enhanced profile and suggest potential applications in phytoremediation. Keywords: biofilm control; systems biology; cross-functional framework; mycoremediation Funding: This work was supported by grants from National Science Foundation (NSF), National Science Foundation (NSF). Discussion: The discovery of innovative scaffold opens up new avenues for research in biosensors and bioelectronics, particularly in the context of biofuel production. Future investigations should address the limitations of our study, such as in silico design using next-generation sequencing.%!(EXTRA string=genome-scale modeling, string=artificial photosynthesis, string=protein engineering, string=cross-functional comprehensive scaffold, string=mycoremediation, string=rational design using organoid technology, string=biosensors and bioelectronics, string=automated signature, string=Halobacterium salinarum, string=enhanced evolving platform, string=industrial biotechnology, string=metabolic engineering, string=groundbreaking paradigm)
相关实验
一、SD大鼠的麻醉 1、SD大鼠腹腔麻醉 把异氟烷和脂肪乳混合做成乳化异氟烷，腹腔注射，3分钟麻醉，30分钟清醒。 乳化异氟烷制备：用30%的脂肪乳为载体，在室温下（20℃）应用玻璃瓶法，配制成8%的乳化异氟烷。即将1.2ml的液态异氟烷和21.3ml的30%脂肪乳加入至一个气密性良好的25～30ml玻璃容器内，在振荡器上以2000r/min的速度剧烈震荡30s，静置30s，然后再以2000r/min速度剧烈震荡50min，达到平衡后放置于4℃冰箱保存备用。 乳化异氟烷，大鼠
参与骨组织形成的细胞，由间充质细胞分化而来，骨膜形成后由骨膜的一些细胞分化而成。成骨细胞短柱状，有突起，核圆形，细胞质强嗜碱性。在将要形成骨组织的地方，排列成单层，彼此由突起相连。成骨细胞的主要功能是生成骨组织的纤维和有机基质。在生成有机的细胞间质以后，本身被埋于其中，变为成骨细胞。这时尚无骨盐，称类骨质。随后，有大量骨盐沉积在有机的细胞间质中，即成为骨组织。
造骨细胞 osteoblast 亦称成骨细胞。是参与骨组织形成的细胞。排列在骨化部位的表面，不久，其本身被骨基质所包埋，形成骨细胞。胚胎的造骨细胞由间充质细胞肥大转化而来。在成体的造骨细胞则是由成纤维细胞或细网细胞肥大转化而来的细胞，以树枝状突起互相连接。在细胞质中含有较多的核糖核酸，呈嗜碱性。骨形成终了后，骨膜内面也有造骨细胞存在，老化的骨则造骨细胞数目减少，随着细胞转化的同时，伴有核蛋白体的减少，嗜碱性几乎消失，线粒体也寥寥无几了。
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大鼠骨细胞
￥1800 - 3800
