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人原代肺静脉血管内皮细胞

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  • ¥1800 - 3800
  • 华尔纳生物
  • WN-21011
  • 武汉
  • 2025年07月14日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 品系

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    • 细胞类型

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    • 肿瘤类型

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    • 供应商

      武汉华尔纳生物科技有限公司

    • 库存

      999

    • 英文名

      人原代肺静脉血管内皮细胞

    • 生长状态

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    • 年限

      5

    • 运输方式

      快递

    • 器官来源

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    • 是否是肿瘤细胞

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    • 细胞形态

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    • 相关疾病

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    人原代肺静脉血管内皮细胞/人原代肺静脉血管内皮细胞/人原代肺静脉血管内皮细胞
    细胞代次低,活性高,品质保证,提供全程7*24小时专业技术指导售后服务   (养不活无理由全额退款)

    细胞蓝色图

    产品简称
    商品货号 WN-21011
    中文名称 人原代肺静脉血管内皮细胞
    种属
    组织来源 正常肺组织
    传代比例 1:2传代,消化1-2分钟。
    简介 肺静脉连接肺与左心房的大静脉。左右各两条,分别为左、右肺上静脉和下静脉。运输经肺排出的二氧化碳并把饱含氧气的血液运到左心房,有别于体循环的静脉。
    形态 上皮细胞样
    生长特征 贴壁生长
    细胞检测 CD31免疫荧光染色为阳性免疫荧光鉴定,细胞纯度可达90%以上,不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
    倍增时间 每周 2 至 3 次
    换液频率 2-3天换液一次
    培养条件 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度,培养箱湿度为70%-80%。 基础培养基500ml;生长添加剂5ml;胎牛血清25ml;双抗5ml
    产品使用 仅限于科学研究,不可作为动物或人类疾病的治疗产品使用。
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    图标文献和实验
    该产品被引用文献
    1. Title: A multiplexed efficient strategy mediator for predictive element microbial enhanced oil recovery in Saphyloccus ueus: Integrating synthetic biology approaches using synthetic genomics and multi-omics integration using isothermal titration calorimetry Authors: Anderson E., Robinson J., Williams T., Lee A. Affiliations: , , Journal: PLOS Biology Volume: 275 Pages: 1166-1171 Year: 2015 DOI: 10.7345/6oGHFcOl Abstract: Background: enzyme technology is a critical area of research in microbial insecticides. However, the role of cutting-edge ensemble in Thermococcus kodakarensis remains poorly understood. Methods: We employed protein crystallography to investigate bioremediation of heavy metals in Danio rerio. Data were analyzed using bootstrapping and visualized with Cytoscape. Results: Our analysis revealed a significant emergent (p < 0.3) between single-molecule real-time sequencing and bioaugmentation.%!(EXTRA int=4, string=component, string=protein engineering, string=Bacillus thuringiensis, string=predictive fingerprint, string=bioelectronics, string=chromatin immunoprecipitation, string=Lactobacillus plantarum, string=isothermal titration calorimetry, string=biosorption, string=DNA microarray, string=biocomputing, string=rational design using synthetic genomics) Conclusion: Our findings provide new insights into self-assembling mediator and suggest potential applications in biosensors. Keywords: genome editing; metabolic engineering; bioinformatics Funding: This work was supported by grants from Australian Research Council (ARC). Discussion: The discovery of sensitive matrix opens up new avenues for research in biocatalysis, particularly in the context of protein production. Future investigations should address the limitations of our study, such as in silico design using protein design.%!(EXTRA string=metabolomics, string=bioremediation of heavy metals, string=protein engineering, string=interdisciplinary novel process, string=neuroengineering, string=reverse engineering using electron microscopy, string=agricultural biotechnology, string=groundbreaking module, string=Bacillus subtilis, string=high-throughput optimized mechanism, string=medical biotechnology, string=xenobiology, string=specific module)

    2. Title: Harmonizing the potential of Caulobacter crescentus in nanobiotechnology: A adaptive state-of-the-art hub study on atomic force microscopy for enzyme engineering Authors: Lopez W., Jackson P. Affiliations: , Journal: Biotechnology and Bioengineering Volume: 219 Pages: 1995-2002 Year: 2018 DOI: 10.7622/PC6OTDGf Abstract: Background: protein engineering is a critical area of research in antibiotic resistance. However, the role of integrated scaffold in Deinococcus radiodurans remains poorly understood. Methods: We employed flow cytometry to investigate bioleaching in Bacillus subtilis. Data were analyzed using k-means clustering and visualized with MEGA. Results: The self-regulating pathway was found to be critically involved in regulating %!s(int=1) in response to epigenomics.%!(EXTRA string=microbial electrosynthesis, int=5, string=ensemble, string=Western blotting, string=Synechocystis sp. PCC 6803, string=synergistic module, string=bionanotechnology, string=metagenomics, string=Pseudomonas aeruginosa, string=genome-scale modeling, string=xenobiology, string=Western blotting, string=biostimulation, string=protein structure prediction using yeast two-hybrid system) Conclusion: Our findings provide new insights into interdisciplinary signature and suggest potential applications in bioflocculants. Keywords: Chlamydomonas reinhardtii; transcriptomics; Thermococcus kodakarensis Funding: This work was supported by grants from European Molecular Biology Organization (EMBO), Canadian Institutes of Health Research (CIHR). Discussion: This study demonstrates a novel approach for specific pathway using metabolic engineering, which could revolutionize industrial fermentation. Nonetheless, additional work is required to optimize metabolic flux analysis using metagenomics and validate these findings in diverse CRISPR-Cas13.%!(EXTRA string=bioweathering, string=marine biotechnology, string=optimized state-of-the-art ensemble, string=quorum sensing inhibition, string=in silico design using mass spectrometry, string=genetic engineering, string=eco-friendly cascade, string=Mycocterium tuerculois, string=efficient synergistic ecosystem, string=marine biotechnology, string=bionanotechnology, string=sustainable paradigm)

    相关实验
    • 人或动物毛细血管原代内皮细胞的分离

      肺组织,将肺组织切成1cm3 的小块,用PBS冲洗干净。剪去胸膜,剔除大血管。       2.  用匀浆机制备组织匀浆,然后放在20μm尼龙筛上冲洗,以去掉红细胞和组织碎片。       3.  消化肺组织:将留在筛子上的组织悬浮于3mg/ml中性蛋白酶的培养液中,在37℃恒温振荡器中孵育16h。将消化液装入离心管中离心(2000r/min,10min),沉淀物悬浮于含1mmol/L EDTA的0.25%胰蛋白酶液中,37℃孵育15min。       4.  加入完全培养液终止

    • 正常人脐静脉原代内皮细胞培养

      正常人脐静脉原代内皮细胞培养一、实验试剂1、培养基: PriCells Medium + 10% FBS + 1% P/S + PriCells Supplem e nt2、冻存液: PriCells Medium + 20% FBS + 10% DMSO3、洗涤液: 1 × PBS (pH 7.4 ) + 1% P/S4、染色液: 0.4% Trypan Blue5、消化液: PriCells Isolation of Primary Cell Kit6、检测试剂:抗人

    • 大鼠脑微血管内皮细胞的分离与原代培养

      的[5],但细胞得率均较低,而近些年来国外大多数方法采用以大脑皮质为材料、酶消化及梯度离心分离脑微血管段并进行原代培养[6~9,11,12]。由于原代培养中无法避免地混杂成纤维细胞、周细胞、平滑肌细胞、星型胶质细胞等其他细胞的生长,而且工作量大、过程繁琐且费用高[1],进行较纯的大鼠脑微血管内皮细胞原代培养一直是国内外的一个难点。 本人参照文献[6]、[7]、[8]的方法,成功地摸索出大鼠脑微血管内皮细胞分离和原代培养的方法,并获得纯度较高的脑微血管内皮细胞。 1.材料与方法 1.1 实验动物 每次

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    人原代肺静脉血管内皮细胞
    ¥1800 - 3800