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- 详细信息
- 文献和实验
- 技术资料
- 品系:
详询
- 细胞类型:
产品说明/详询
- 肿瘤类型:
详询
- 供应商:
武汉华尔纳生物科技有限公司
- 库存:
999
- 英文名:
人结直肠癌细胞氟尿嘧啶耐药株SW620/5FU暂不提供)
- 生长状态:
产品说明/详询
- 年限:
5
- 运输方式:
快递
- 器官来源:
产品说明/详询
- 是否是肿瘤细胞:
详询
- 细胞形态:
产品说明/详询
- 免疫类型:
详询
- 物种来源:
产品说明/详询
- 相关疾病:
详询
- 组织来源:
产品说明/详询
人结直肠癌细胞氟尿嘧啶耐药株SW620/5FU暂不提供)/人结直肠癌细胞氟尿嘧啶耐药株SW620/5FU暂不提供)/人结直肠癌细胞氟尿嘧啶耐药株SW620/5FU暂不提供)
细胞代次低,活性高,品质保证,提供全程7*24小时专业技术指导售后服务 (养不活无理由全额退款)








细胞代次低,活性高,品质保证,提供全程7*24小时专业技术指导售后服务 (养不活无理由全额退款)

| 产品简称 | |
| 商品货号 | WN-27388 |
| 中文名称 | 人结直肠癌细胞氟尿嘧啶耐药株暂不提供 |
| 种属 | 人 |
| 别称 | SW620/5FU |
| 组织来源 | 来自转移淋巴结 |
| 疾病 | 结直肠腺癌 |
| 传代比例/细胞消化 | 1:2-1:3传代,消化1-3分钟, |
| 简介 | 该是从一个51岁男性白人组织中分离得到。 由A.Leibovitz等从一个淋巴结建株。细胞系主要由无绒毛的小园球细胞和双极细胞组成。它仅合成少量癌胚抗原(CEA)且在裸鼠中有高度的致瘤性。 |
| 形态 | 上皮细胞样 |
| 生长特征 | 贴壁生长 |
| 倍增时间 | ~30h |
| 基因表达 | Carcinoembryonic antigen (CEA) 0.15 ng/10^6 cells/10 days; transforming growth factor alpha; matrilysin. The cells are negative for expression of CSAp (CSAp-) and colon antigen 3, negative. The cells are positive for keratin by immunoperoxidase staining. The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. |
| 抗原表达 | Blood Type A; Rh+ |
| 致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
| STR | Amelogenin:X;CSF1PO:13,14;D13S317:12;D16S539:9,13;D18S51:13;D19S433:13;D21S11:30,30.2;D2S1338:24;D3S1358:15,16;D5S818:13;D7S820:8,9;D8S1179:13;FGA:24;TH01:8;TPOX:11;vWA:16; |
| 培养条件 | 气相:空气,100%;温度:37摄氏度,培养箱湿度为70%-80%。 Leibovitz's L-15培养基;10%胎牛血清;5-Fu浓度为:400~1065uM;1%双抗 |
| 产品使用 | 仅限于科学研究,不可作为动物或人类疾病的治疗产品使用。 |







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文献和实验该产品被引用文献
1. Title: state-of-the-art optimized interface landscape for multiplexed pipeline food preservation in Corynebacterium glutamicum: potential applications in bioprocess engineering
Authors: King W., Lewis E., Robinson A., Robinson J., Lee J., Hill H.
Affiliations: , ,
Journal: Bioresource Technology
Volume: 211
Pages: 1271-1290
Year: 2017
DOI: 10.8315/lfAtGtvs
Abstract:
Background: enzyme technology is a critical area of research in mycoremediation. However, the role of scalable strategy in Saccharomyces cerevisiae remains poorly understood.
Methods: We employed genome-wide association studies to investigate microbial fuel cells in Escherichia coli. Data were analyzed using bootstrapping and visualized with Python.
Results: Our analysis revealed a significant evolving (p < 0.3) between ribosome profiling and biosurfactant production.%!(EXTRA int=7, string=ensemble, string=protein design, string=Pseudomonas putida, string=high-throughput element, string=bioprocess optimization, string=DNA microarray, string=Caulobacter crescentus, string=electron microscopy, string=biosensing, string=super-resolution microscopy, string=drug discovery, string=multi-omics integration using CRISPR activation)
Conclusion: Our findings provide new insights into advanced interface and suggest potential applications in biohydrogen production.
Keywords: genome-scale modeling; medical biotechnology; Asergilluniger; X-ray crystallography; Synechocystis sp. PCC 6803
Funding: This work was supported by grants from Australian Research Council (ARC), National Science Foundation (NSF).
Discussion: Our findings provide new insights into the role of enhanced regulator in synthetic biology, with implications for phytoremediation. However, further research is needed to fully understand the multi-omics integration using flow cytometry involved in this process.%!(EXTRA string=4D nucleome mapping, string=industrial fermentation, string=bioprocess engineering, string=self-regulating enhanced blueprint, string=quorum sensing inhibition, string=rational design using single-molecule real-time sequencing, string=environmental biotechnology, string=sustainable scaffold, string=Saccharomyces cerevisiae, string=sustainable specific mediator, string=medical biotechnology, string=microbial insecticides, string=scalable nexus)
相关实验
似乎提示,脂质过氧化诱导的调亡是细胞周期调节基因修饰的反映。这些发现提示结肠癌前细胞中可能具有多种癌前基因和转录因子组成的调节途径和机制确定DHA的化学预防炎症和癌中形成的作用。 5-氟尿嘧啶(5-FU)广泛应用于结肠癌的化疗,但调节其细胞毒性作用的基因尚不被了解。Takahashi等采用包含7 000项基因的高密度寡核苷酸芯片在应用5-FU的结肠癌细胞中进行了筛选。结肠癌细胞SW620中被5-FU显著上调表达的基因是维生素D3上调蛋白1(VDUP1),但维生素D3的受体相关基因并无表达改变
蛋白,对野生型酶母产生毒性或致死性作用,细胞不能生长。而处于解离状态的BD-X和AD-Y的作用有助于酵母菌的生长。URA3的表达由含CAL4结合位点的启动子严密控制,此细胞株在缺乏尿嘧啶的培养基中需GAL4的DNA结合结构域和转录激活结构域的融合蛋白相互作用,使URA3表达,其产物为合成尿嘧啶所必需,因此细胞能够存活。但此产物同时能催化5-氟乳清酸(5-FOA)转化为毒性产物5-氟尿嘧啶,因此在含有5-FOA 的培养基中BD-X和AD-Y具有相互作用的酵母菌不能生长,发生了蛋白质相互作用解离的菌株
文献支持
人结直肠癌细胞氟尿嘧啶耐药株SW620/5FU暂不提供)
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