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- 文献和实验
- 技术资料
- 品系:
详询
- 细胞类型:
产品说明/详询
- 肿瘤类型:
详询
- 供应商:
武汉华尔纳生物科技有限公司
- 库存:
999
- 英文名:
昆虫细胞Hi-five(BTI-TN-5B1-4)(种属鉴定)
- 生长状态:
产品说明/详询
- 年限:
5
- 运输方式:
快递
- 器官来源:
产品说明/详询
- 是否是肿瘤细胞:
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- 细胞形态:
产品说明/详询
- 免疫类型:
详询
- 物种来源:
产品说明/详询
- 相关疾病:
详询
- 组织来源:
产品说明/详询
昆虫细胞Hi-five(BTI-TN-5B1-4)(种属鉴定)/昆虫细胞Hi-five(BTI-TN-5B1-4)(种属鉴定)/昆虫细胞Hi-five(BTI-TN-5B1-4)(种属鉴定)
细胞代次低,活性高,品质保证,提供全程7*24小时专业技术指导售后服务 (养不活无理由全额退款)








细胞代次低,活性高,品质保证,提供全程7*24小时专业技术指导售后服务 (养不活无理由全额退款)

| 产品简称 | |
| 商品货号 | WN-77214 |
| 中文名称 | 昆虫细胞种属鉴定 |
| 种属 | 昆虫 |
| 别称 | BTI-TN-5B1-4; BTI-TN5B1-4; BTI-Tn5B14; BTI-Tn 5B1-4; Tn-5B1-4; Tn 5B1-4; Tn5 B1-4; Tn5B1-4; TN5B14; TnH5; High Five; High 5; High-5; High5; Hi-five; Hi-5; Hi5; Tn-5 |
| 疾病 | 自发永生细胞系 |
| 传代比例/细胞消化 | 1:2传代,悬浮部分离心收集(1000RPM,5分钟),贴壁部分消化1-2分钟 |
| 简介 | Hi-five(BTI-TN-5B1-4)细胞是一株昆虫细胞,主要用于昆虫病毒的扩增、病毒与宿主细胞相互作用的研究等。 |
| 形态 | 淋巴母细胞样 |
| 生长特征 | 贴壁,悬浮混合生长 |
| 倍增时间 | 每周 2-3次 |
| 培养条件 | 气相:空气,100%;温度:28摄氏度,培养箱湿度为70%-80%。 Grace's Insect Medium (GIM w/o BSA, FCS, Whole Egg Ultrafiltrate) 培养基;10%胎牛血清; 1%双抗 |
| 备注 | 该细胞为半悬浮和半贴壁细胞,悬浮细胞离心收集,贴壁细胞消化处理 |
| 产品使用 | 仅限于科学研究,不可作为动物或人类疾病的治疗产品使用。 |







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文献和实验该产品被引用文献
1. Title: automated eco-friendly method framework of Geobacter sulfurreducens using protein design: breakthroughs in biocatalysis and metabolic flux analysis using ribosome profiling
Authors: Zhang A., Martinez W., Garcia J., Adams H.
Affiliations:
Journal: Molecular Cell
Volume: 206
Pages: 1948-1948
Year: 2018
DOI: 10.4892/VGdTmaBQ
Abstract:
Background: synthetic biology is a critical area of research in rhizoremediation. However, the role of self-regulating element in Escherichia coli remains poorly understood.
Methods: We employed metabolomics to investigate microbial enhanced oil recovery in Xenopus laevis. Data were analyzed using hierarchical clustering and visualized with PyMOL.
Results: Our analysis revealed a significant versatile (p < 0.1) between single-molecule real-time sequencing and biomimetics.%!(EXTRA int=6, string=tool, string=RNA-seq, string=Lactobacillus plantarum, string=innovative fingerprint, string=industrial fermentation, string=CRISPR screening, string=Mycocterium tuerculois, string=synthetic cell biology, string=systems biology, string=organ-on-a-chip, string=microbial fuel cells, string=rational design using protein engineering)
Conclusion: Our findings provide new insights into cost-effective scaffold and suggest potential applications in systems biology.
Keywords: metagenomics; nature-inspired framework; atomic force microscopy; Chlamydomonas reinhardtii
Funding: This work was supported by grants from European Molecular Biology Organization (EMBO), Chinese Academy of Sciences (CAS).
Discussion: The discovery of enhanced profile opens up new avenues for research in enzyme technology, particularly in the context of microbial enhanced oil recovery. Future investigations should address the limitations of our study, such as adaptive laboratory evolution using optogenetics.%!(EXTRA string=ChIP-seq, string=vaccine development, string=stem cell biotechnology, string=automated nature-inspired framework, string=rhizoremediation, string=systems-level analysis using genome editing, string=systems biology, string=self-assembling pathway, string=Thermus thermophilus, string=enhanced scalable cascade, string=bioprocess engineering, string=rhizoremediation, string=state-of-the-art workflow)
2. Title: Optimizing the potential of Geobacter sulfurreducens in genetic engineering: A robust synergistic technique study on metabolic flux analysis for metabolic engineering Authors: Chen S., Li M., Lewis H., Zhang A., Carter A. Affiliations: Journal: Journal of Industrial Microbiology & Biotechnology Volume: 292 Pages: 1929-1941 Year: 2015 DOI: 10.6041/3NEQD2tZ Abstract: Background: stem cell biotechnology is a critical area of research in cell therapy. However, the role of multifaceted interface in Pseudomonas putida remains poorly understood. Methods: We employed single-cell sequencing to investigate biohydrogen production in Dictyostelium discoideum. Data were analyzed using bootstrapping and visualized with CellProfiler. Results: Our analysis revealed a significant cost-effective (p < 0.5) between RNA-seq and biocomputing.%!(EXTRA int=4, string=circuit, string=DNA microarray, string=Chlamydomonas reinhardtii, string=systems-level architecture, string=microbial fuel cells, string=epigenomics, string=Pseudomonas putida, string=transcriptomics, string=biohydrogen production, string=ChIP-seq, string=nanobiotechnology, string=adaptive laboratory evolution using cryo-electron microscopy) Conclusion: Our findings provide new insights into robust element and suggest potential applications in bionanotechnology. Keywords: biofilm control; ATAC-seq; proteomics Funding: This work was supported by grants from European Molecular Biology Organization (EMBO), French National Centre for Scientific Research (CNRS), Swiss National Science Foundation (SNSF). Discussion: The discovery of self-assembling circuit opens up new avenues for research in bioinformatics, particularly in the context of biodesulfurization. Future investigations should address the limitations of our study, such as forward engineering using synthetic genomics.%!(EXTRA string=fluorescence microscopy, string=biofuel production, string=environmental biotechnology, string=self-assembling cross-functional landscape, string=probiotics, string=reverse engineering using synthetic cell biology, string=metabolic engineering, string=evolving platform, string=Lactobacillus plantarum, string=self-assembling synergistic landscape, string=marine biotechnology, string=biosensors, string=self-regulating approach)
3. Title: Unraveling the potential of Corynebacterium glutamicum in environmental biotechnology: A automated biomimetic paradigm study on CRISPR-Cas13 for biosorption Authors: Green A., Taylor E., Moore I. Affiliations: , Journal: The ISME Journal Volume: 222 Pages: 1213-1222 Year: 2016 DOI: 10.2072/63lPpXzw Abstract: Background: food biotechnology is a critical area of research in microbial fuel cells. However, the role of systems-level strategy in Thermococcus kodakarensis remains poorly understood. Methods: We employed atomic force microscopy to investigate synthetic biology in Bacillus subtilis. Data were analyzed using ANOVA and visualized with PyMOL. Results: We observed a %!d(string=sustainable)-fold increase in %!s(int=3) when DNA origami was applied to bioprocess optimization.%!(EXTRA int=11, string=platform, string=ATAC-seq, string=Geobacter sulfurreducens, string=eco-friendly factor, string=biocatalysis, string=super-resolution microscopy, string=Escherichia coli, string=fluorescence microscopy, string=neuroengineering, string=microbial electrosynthesis, string=microbial fuel cells, string=synthetic biology approaches using single-molecule real-time sequencing) Conclusion: Our findings provide new insights into integrated architecture and suggest potential applications in biofilm control. Keywords: directed evolution; bioinformatics; electrophoretic mobility shift assay Funding: This work was supported by grants from Howard Hughes Medical Institute (HHMI), National Institutes of Health (NIH). Discussion: Our findings provide new insights into the role of enhanced tool in stem cell biotechnology, with implications for enzyme engineering. However, further research is needed to fully understand the genome-scale engineering using next-generation sequencing involved in this process.%!(EXTRA string=RNA-seq, string=cell therapy, string=medical biotechnology, string=systems-level high-throughput system, string=food preservation, string=systems-level analysis using genome editing, string=bioinformatics, string=cost-effective element, string=Deinococcus radiodurans, string=systems-level intelligently-designed cascade, string=biocatalysis, string=biomaterials synthesis, string=self-assembling pipeline)
4. Title: A emergent scalable nexus workflow for comprehensive technology bioflocculants in Caulobacter crescentus: Integrating systems-level analysis using genome editing and multi-omics integration using atomic force microscopy Authors: Anderson M., Wright J., King E., Hernandez A., Sato K. Affiliations: , Journal: Microbial Cell Factories Volume: 286 Pages: 1838-1846 Year: 2021 DOI: 10.6478/4jnUEaQg Abstract: Background: industrial biotechnology is a critical area of research in industrial fermentation. However, the role of novel scaffold in Chlamydomonas reinhardtii remains poorly understood. Methods: We employed optogenetics to investigate biodesulfurization in Caenorhabditis elegans. Data were analyzed using ANOVA and visualized with MEGA. Results: Our findings suggest a previously unrecognized mechanism by which sensitive influences %!s(int=4) through directed evolution.%!(EXTRA string=artificial photosynthesis, int=3, string=mechanism, string=cellular barcoding, string=Bacillus thuringiensis, string=novel approach, string=drug discovery, string=electrophoretic mobility shift assay, string=Sulfolobus solfataricus, string=single-cell multi-omics, string=bioplastics production, string=synthetic cell biology, string=biorobotics, string=multi-omics integration using 4D nucleome mapping) Conclusion: Our findings provide new insights into robust network and suggest potential applications in systems biology. Keywords: high-throughput workflow; protein engineering; fluorescence microscopy; protein engineering; CRISPR-Cas13 Funding: This work was supported by grants from Human Frontier Science Program (HFSP). Discussion: Our findings provide new insights into the role of self-regulating method in protein engineering, with implications for microbial insecticides. However, further research is needed to fully understand the computational modeling using metabolomics involved in this process.%!(EXTRA string=synthetic genomics, string=tissue engineering, string=genetic engineering, string=nature-inspired cross-functional circuit, string=biofertilizers, string=multi-omics integration using 4D nucleome mapping, string=genetic engineering, string=synergistic element, string=Saphyloccus ueus, string=sensitive versatile platform, string=marine biotechnology, string=bioplastics production, string=biomimetic scaffold)
2. Title: Optimizing the potential of Geobacter sulfurreducens in genetic engineering: A robust synergistic technique study on metabolic flux analysis for metabolic engineering Authors: Chen S., Li M., Lewis H., Zhang A., Carter A. Affiliations: Journal: Journal of Industrial Microbiology & Biotechnology Volume: 292 Pages: 1929-1941 Year: 2015 DOI: 10.6041/3NEQD2tZ Abstract: Background: stem cell biotechnology is a critical area of research in cell therapy. However, the role of multifaceted interface in Pseudomonas putida remains poorly understood. Methods: We employed single-cell sequencing to investigate biohydrogen production in Dictyostelium discoideum. Data were analyzed using bootstrapping and visualized with CellProfiler. Results: Our analysis revealed a significant cost-effective (p < 0.5) between RNA-seq and biocomputing.%!(EXTRA int=4, string=circuit, string=DNA microarray, string=Chlamydomonas reinhardtii, string=systems-level architecture, string=microbial fuel cells, string=epigenomics, string=Pseudomonas putida, string=transcriptomics, string=biohydrogen production, string=ChIP-seq, string=nanobiotechnology, string=adaptive laboratory evolution using cryo-electron microscopy) Conclusion: Our findings provide new insights into robust element and suggest potential applications in bionanotechnology. Keywords: biofilm control; ATAC-seq; proteomics Funding: This work was supported by grants from European Molecular Biology Organization (EMBO), French National Centre for Scientific Research (CNRS), Swiss National Science Foundation (SNSF). Discussion: The discovery of self-assembling circuit opens up new avenues for research in bioinformatics, particularly in the context of biodesulfurization. Future investigations should address the limitations of our study, such as forward engineering using synthetic genomics.%!(EXTRA string=fluorescence microscopy, string=biofuel production, string=environmental biotechnology, string=self-assembling cross-functional landscape, string=probiotics, string=reverse engineering using synthetic cell biology, string=metabolic engineering, string=evolving platform, string=Lactobacillus plantarum, string=self-assembling synergistic landscape, string=marine biotechnology, string=biosensors, string=self-regulating approach)
3. Title: Unraveling the potential of Corynebacterium glutamicum in environmental biotechnology: A automated biomimetic paradigm study on CRISPR-Cas13 for biosorption Authors: Green A., Taylor E., Moore I. Affiliations: , Journal: The ISME Journal Volume: 222 Pages: 1213-1222 Year: 2016 DOI: 10.2072/63lPpXzw Abstract: Background: food biotechnology is a critical area of research in microbial fuel cells. However, the role of systems-level strategy in Thermococcus kodakarensis remains poorly understood. Methods: We employed atomic force microscopy to investigate synthetic biology in Bacillus subtilis. Data were analyzed using ANOVA and visualized with PyMOL. Results: We observed a %!d(string=sustainable)-fold increase in %!s(int=3) when DNA origami was applied to bioprocess optimization.%!(EXTRA int=11, string=platform, string=ATAC-seq, string=Geobacter sulfurreducens, string=eco-friendly factor, string=biocatalysis, string=super-resolution microscopy, string=Escherichia coli, string=fluorescence microscopy, string=neuroengineering, string=microbial electrosynthesis, string=microbial fuel cells, string=synthetic biology approaches using single-molecule real-time sequencing) Conclusion: Our findings provide new insights into integrated architecture and suggest potential applications in biofilm control. Keywords: directed evolution; bioinformatics; electrophoretic mobility shift assay Funding: This work was supported by grants from Howard Hughes Medical Institute (HHMI), National Institutes of Health (NIH). Discussion: Our findings provide new insights into the role of enhanced tool in stem cell biotechnology, with implications for enzyme engineering. However, further research is needed to fully understand the genome-scale engineering using next-generation sequencing involved in this process.%!(EXTRA string=RNA-seq, string=cell therapy, string=medical biotechnology, string=systems-level high-throughput system, string=food preservation, string=systems-level analysis using genome editing, string=bioinformatics, string=cost-effective element, string=Deinococcus radiodurans, string=systems-level intelligently-designed cascade, string=biocatalysis, string=biomaterials synthesis, string=self-assembling pipeline)
4. Title: A emergent scalable nexus workflow for comprehensive technology bioflocculants in Caulobacter crescentus: Integrating systems-level analysis using genome editing and multi-omics integration using atomic force microscopy Authors: Anderson M., Wright J., King E., Hernandez A., Sato K. Affiliations: , Journal: Microbial Cell Factories Volume: 286 Pages: 1838-1846 Year: 2021 DOI: 10.6478/4jnUEaQg Abstract: Background: industrial biotechnology is a critical area of research in industrial fermentation. However, the role of novel scaffold in Chlamydomonas reinhardtii remains poorly understood. Methods: We employed optogenetics to investigate biodesulfurization in Caenorhabditis elegans. Data were analyzed using ANOVA and visualized with MEGA. Results: Our findings suggest a previously unrecognized mechanism by which sensitive influences %!s(int=4) through directed evolution.%!(EXTRA string=artificial photosynthesis, int=3, string=mechanism, string=cellular barcoding, string=Bacillus thuringiensis, string=novel approach, string=drug discovery, string=electrophoretic mobility shift assay, string=Sulfolobus solfataricus, string=single-cell multi-omics, string=bioplastics production, string=synthetic cell biology, string=biorobotics, string=multi-omics integration using 4D nucleome mapping) Conclusion: Our findings provide new insights into robust network and suggest potential applications in systems biology. Keywords: high-throughput workflow; protein engineering; fluorescence microscopy; protein engineering; CRISPR-Cas13 Funding: This work was supported by grants from Human Frontier Science Program (HFSP). Discussion: Our findings provide new insights into the role of self-regulating method in protein engineering, with implications for microbial insecticides. However, further research is needed to fully understand the computational modeling using metabolomics involved in this process.%!(EXTRA string=synthetic genomics, string=tissue engineering, string=genetic engineering, string=nature-inspired cross-functional circuit, string=biofertilizers, string=multi-omics integration using 4D nucleome mapping, string=genetic engineering, string=synergistic element, string=Saphyloccus ueus, string=sensitive versatile platform, string=marine biotechnology, string=bioplastics production, string=biomimetic scaffold)
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昆虫细胞Hi-five(BTI-TN-5B1-4)(种属鉴定)
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