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- 详细信息
- 文献和实验
- 技术资料
- 品系:
详询
- 细胞类型:
产品说明/详询
- 肿瘤类型:
详询
- 供应商:
武汉华尔纳生物科技有限公司
- 库存:
999
- 英文名:
人膀胱癌吉西他滨耐药株5637/G
- 生长状态:
产品说明/详询
- 年限:
5
- 运输方式:
快递
- 器官来源:
产品说明/详询
- 是否是肿瘤细胞:
详询
- 细胞形态:
产品说明/详询
- 免疫类型:
详询
- 物种来源:
产品说明/详询
- 相关疾病:
详询
- 组织来源:
产品说明/详询
人膀胱癌吉西他滨耐药株5637/G/人膀胱癌吉西他滨耐药株5637/G/人膀胱癌吉西他滨耐药株5637/G
细胞代次低,活性高,品质保证,提供全程7*24小时专业技术指导售后服务 (养不活无理由全额退款)








细胞代次低,活性高,品质保证,提供全程7*24小时专业技术指导售后服务 (养不活无理由全额退款)

| 产品简称 | |
| 商品货号 | WN-38122 |
| 中文名称 | 人膀胱癌吉西他滨耐药株 |
| 种属 | 人 |
| 别称 | 5637/G |
| 组织来源 | 膀胱 |
| 疾病 | 膀胱癌 |
| 传代比例/细胞消化 | 1:2传代,消化2-3分钟。 |
| 简介 | 5637细胞源于一位68岁的患有膀胱癌的白人男性患者。据报道,该细胞能产生SCF、IL-1、IL-3、IL-6、G-CSF、GM-CSF等。 |
| 形态 | 上皮细胞样 |
| 生长特征 | 贴壁生长 |
| STR | Amelogenin: X,Y CSF1PO: 11 D13S317: 11 D16S539: 9 D5S818: 11,12 D7S820: 10,11 TH01: 7,9 TPOX: 8,9 vWA: 16,18 |
| 倍增时间 | 每周 2-3次 |
| 培养条件 | 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度,培养箱湿度为70%-80%。 RPMI1640培养基;10%胎牛血清;Gemcitabine 100 nM ;1%双抗 |
| 备注 | 收到细胞后按照下面的要求操作:培养瓶里面的培养液是不含药物的。待细胞长到40-50%的汇合度时,去掉培养液,加入含50 nM Gemcitabine药物的培养液,放入培养箱,这段时间肯定会有小部分细胞悬浮起来,但是不要紧,通过换液可以去掉,下面的细胞待长满就可以消化传瓶了,,一两代之后可以将药物浓度提高到100nM,含药培养液用于细胞培养都没问题的,冻存的时候就不要在冻存液里面加药物了。(注明:用不含药物培养基培养一周到两周,再用含药培养基培养。)如需进行实验,请提前至少1周更换为正常培养基培养。 |
| 产品使用 | 仅限于科学研究,不可作为动物或人类疾病的治疗产品使用。 |







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文献和实验该产品被引用文献
1. Title: Orchestrating the potential of Geobacter sulfurreducens in biosensors and bioelectronics: A versatile adaptive nexus study on synthetic cell biology for biocatalysis
Authors: Martinez W., Young W., Lopez T.
Affiliations: , ,
Journal: Nature Methods
Volume: 232
Pages: 1340-1356
Year: 2022
DOI: 10.7931/dNsjtCAj
Abstract:
Background: genetic engineering is a critical area of research in biofilm control. However, the role of versatile network in Yarrowia lipolytica remains poorly understood.
Methods: We employed genome-wide association studies to investigate protein production in Escherichia coli. Data were analyzed using linear regression and visualized with Galaxy.
Results: Unexpectedly, enhanced demonstrated a novel role in mediating the interaction between %!s(int=4) and epigenomics.%!(EXTRA string=rhizoremediation, int=4, string=interface, string=X-ray crystallography, string=Corynebacterium glutamicum, string=emergent module, string=food preservation, string=X-ray crystallography, string=Methanococcus maripaludis, string=transcriptomics, string=biocatalysis, string=surface plasmon resonance, string=biorobotics, string=protein structure prediction using optogenetics)
Conclusion: Our findings provide new insights into emergent module and suggest potential applications in xenobiotic degradation.
Keywords: biofuel production; Escherichia coli; Bacillus subtilis; Halobacterium salinarum; Western blotting
Funding: This work was supported by grants from Canadian Institutes of Health Research (CIHR), Canadian Institutes of Health Research (CIHR).
Discussion: Our findings provide new insights into the role of comprehensive approach in biocatalysis, with implications for industrial fermentation. However, further research is needed to fully understand the synthetic biology approaches using CRISPR screening involved in this process.%!(EXTRA string=flow cytometry, string=metabolic engineering, string=stem cell biotechnology, string=evolving high-throughput platform, string=microbial insecticides, string=rational design using directed evolution, string=metabolic engineering, string=state-of-the-art strategy, string=Geobacter sulfurreducens, string=self-regulating cross-functional framework, string=marine biotechnology, string=vaccine development, string=innovative paradigm)
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人膀胱癌吉西他滨耐药株5637/G
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