雷帕霉素 *Sigma货号：R0395-1MG

一般描述

化学结构：大环内酯 Rapamycin is derived from Streptomyces hygroscopicus . It can inhibit viral replication. This natural anti-fungal antibiotic is a proven anti-aging agent. Rapamycin significantly inhibits mTORC1 regardless of age.[1] It suppresses protein kinase C activity and enhances ion transport in A6 cells. It also suppresses the immune response in membrane and cytosolic preparations.[2] Additionally, it has specific effects on the translation of endogenous mRNAs and inhibits the translation of 5′TOP mRNAs by blocking p70s6k activation in the signaling pathway. Rapamycin exhibits antineoplastic properties. 应用
Rapamycin from Streptomyces hygroscopicus has been used: to treat BV2 cells or BV2-LC3 cells for autophagy assay[3] as an autophagy activator in the bone marrow mesenchymal stem cells (BM-MSCs) transfected with lentivirus carrier of small interfering RNA for FOXO3 (siFOXO3) to study the effects of Forkhead Box O3 (FOXO3) on the autophagy of BM-MSCs[4] to pre-incubate villi to inhibit the mammalian target of rapamycin complex 1 (mTORC1) signaling[5]

生化/生理作用

一种与雷帕霉素(mTOR)分子靶点结合并抑制其作用的大环三烯抗生素
它与FKBP12形成复合物，结合并抑制雷帕霉素(mTOR)的分子靶点
雷帕霉素是一种有效的免疫抑制剂，并具有抗癌活性