多聚右旋赖氨酸 *Sigma货号：P7405-5MG

一般描述

多聚-D-赖氨酸氢溴suan盐（PDL）是一种赖氨酸残基组成的阳离子聚合物，其中的每个赖氨酸单元结合一个氢溴suan分子
氢溴suan分子的存在赋予多聚-D-赖氨酸独特的性质，使得多聚-D-赖氨酸具有晶体形态并可溶于水性溶液中
应用
Poly-D-lysine hydrobromide salt has been used to treat the surface of glass slides for cell attachment (4) and (5), and to coat glass bottomed culture dishes for live cell imaging of primary cortical neurons. (6) A 24 well plate was used to isolate primary astrocytes and investigate the effect of exosomes derived from melanoma on stromal cell reprogramming in a metastatic microenvironment. (7) Used for coating 16 well chamber glass slides to investigate the effects of maternal immune activation on behavioral disorders and changes in the expression of cytokines and synaptic proteins in the cerebellum. (8) Poly-D-lysine polymer can be used to prepare surfaces for cell attachment. D-Lysine polymer can also be used for cells that digest poly-L-Lysine polymer and cause excessive uptake of L-lysine. It is recommended to use 0.5-1.0 mL of 0.1 mg/mL solution of this product as the substrate for cell culture to coat 25 cm2. The viscosity of the product with lower molecular weight is relatively low, but the higher molecular weight form can provide more attachment sites per molecule

生化/生理作用

多聚-L-赖氨酸氢溴suan盐（PDL）是细胞的非特异性粘附因子，它通过增强细胞膜表面的负电荷离子与培养表面之间的静电相互作用，促进细胞与固相基质的粘附
(3) 在吸附至培养物表面后，PDL增加带正电细胞结合位点的数目
(2) PDL 涂层促进悬浮生长的细胞在固体表面的附着
(1) 多聚-L-赖氨酸（PDL）氢溴suan盐是细胞的非特异性粘附因子，它通过增强细胞膜表面的负电荷离子与培养表面之间的静电相互作用，促进细胞与固相基质的粘附
当被细胞培养表面吸收后，聚-D-赖氨酸可增加带正电的细胞结合位点数量
组分
聚-D-赖氨酸是一种带正电的氨基酸聚合物，其中每个赖氨酸残基约有一个HBr
氢溴suan盐可使聚-D-赖氨酸呈可溶于水的结晶形式
在β结构中可能会发现少量的产物，因为HBr干扰氨基与羧基或含N或O部分之间的氢键