1-乙基-(3-二甲基氨基丙基)碳二亚胺盐酸盐 *Sigma

一般描述

1-乙基-3-（3-二甲基氨基丙基）碳二亚胺盐酸盐（常被称为EDAC HCl）是化学和生物化学研究中的功能强大、应用广泛的水溶性试剂，这一点归因于它在促进酰胺键形成中的关键作用
在肽合成中，EDC HCl通过羧基和胺基与氨基酸高效结合，从而形成肽骨架（peptide backbone）
这种性能对于合成具有特定序列和功能的肽特别有用
除了肽以外，EDC HCl还可用于免疫原的合成，在此过程中它以共价方式使半抗原（小的免疫反应诱导分子）连接载体蛋白，在疫苗研究中起重要作用
EDAC HCl 的多功能性还体现在其修饰核酸的能力，从而通过其5′磷酸基团标记DNA和RNA
这一点有助于这些关键分子的成像、追踪和分析，为核酸研究的进步做出贡献
In addition, EDAC HCl can serve as a biomolecule bridge, acting as a crosslinking agent to connect amine reactive NHS esters and carboxyl groups in biomolecules. This technology has important value in protein coupling and can create hybrid molecules with novel characteristics and functions. The potential mechanism of EDAC HCl involves the formation of unstable intermediates through its reaction with carboxyl groups, which actively attract amine partners. The subtle balance of this reaction highlights the importance of optimizing conditions to achieve efficient coupling. N-hydroxysuccinimide (NHS) further enhances the performance of EDAC HCl, stabilizes intermediates, and achieves a two-step coupling process. This additional feature brings greater flexibility and control, especially in handling complex biomolecules