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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12 months
- 英文名:
Recombinant Wheat Tri a 28/IAA1 Protein, C-His
- 供应商:
武汉益普生物科技有限公司
- 规格:
50μg/100μg/1mg
| 规格: | 50μg | 产品价格: | ¥1600.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥2580.0 |
| 规格: | 1mg | 产品价格: | ¥15990.0 |
| 产品名 | Recombinant Wheat Tri a 28/IAA1 Protein, C-His |
| 货号 | EZZ05601 |
| 别名 | Alpha-amylase inhibitor 0.19, 0.19 alpha-AI, 0.19 AI, Tri a 28 |
| 表达系统 | Insect Cells |
| Accession号 | P01085 |
| 种属 | Triticum aestivum (Wheat) |
| 蛋白长度 | Ser1-Ala124 |
| 预测分子量 | 16.27 kDa |
| 性质 | Recombinant |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 状态 | Lyophilized |
| 保存溶液 | Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 说明 | For research use only. |

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文献和实验X-100或脱氧胆酸钠)和低浓度变性剂(2mol/L尿素或盐酸胍等)洗涤除去脂类和膜蛋白,这一步很重要,否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂,如8mol/L尿素、6~8mol/L盐酸胍,通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差,异氰盐酸胍最强;去污剂,如SDS[7],可以破坏蛋白内的疏水键,可以增溶几乎所有的蛋白,但由于无法彻底去除而不允许用在制药行业中;酸,如70%甲酸[9],可以破坏
Strategies to Optimize Protein Expression in E. coli
., Cantor, E.J., Liao, W., Xu, M.Q., and Benner, J. 1998. Utilizing the C‐terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step. Nucleic Acids Res. 26:5109‐5115
The STINT‐NMR Method for Studying In‐cell Protein‐Protein Interactions
. Cruzeiro‐Silva, C., Albernaz, F.P., Valente, A.P., and Almeida, F.C. 2006. In‐Cell NMR spectroscopy: Inhibition of autologous protein expression reduces Escherichia coli lysis. Cell Biochem. Biophys. 44:497
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