- ￥1280 - 9776
- AntibodySystem
- France
- YWN45301
- 2025年12月06日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12 months
- 英文名：
Recombinant Chinese mitten crab Met Protein, N-His
- 供应商：
武汉益普生物科技有限公司
- 规格：
50μg/100μg/1mg
| 规格： | 50μg | 产品价格： | ￥1280.0 |
|---|---|---|---|
| 规格： | 100μg | 产品价格： | ￥1880.0 |
| 规格： | 1mg | 产品价格： | ￥9776.0 |
| 产品名 | Recombinant Chinese mitten crab Met Protein, N-His |
| 货号 | YWN45301 |
| 别名 | Methoprene-tolerant protein, Met |
| 表达系统 | E. coli |
| Accession号 | A0A7D5R690 |
| 种属 | Eriocheir sinensis (Chinese mitten crab) |
| 蛋白长度 | Ser51-Arg281 |
| 预测分子量 | 27.83 kDa |
| 性质 | Recombinant |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 状态 | Lyophilized |
| 保存溶液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 说明 | For research use only. |
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文献和实验Expression and Purification of Recombinant -Defensins and -Defensin Precursors in Escherichia coli
and expressed in E. coli BL21 RIS cells. Cells growing exponentially in nutrient-rich liquid medium are induced to express the recombinant protein by addition of 50 mM isopropyl β-d -1-thiogalactopyranoside for 3–6 h. After bacterial cells collected
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
Basic Protocol 1: Generation of an Active Recombinant Protein That is End‐Labeled Basic Protocol 2: Hydroxyl Radical Cleavage of Protein in Absence and Presence of Ligand Basic Protocol 3: Analyze Gel
Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
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