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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12 months
- 英文名:
Recombinant Arabidopsis thaliana CSN4 Protein, N-His
- 供应商:
武汉益普生物科技有限公司
- 规格:
50μg/100μg/1mg
| 规格: | 50μg | 产品价格: | ¥1280.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥1880.0 |
| 规格: | 1mg | 产品价格: | ¥9776.0 |
| 产品名 | Recombinant Arabidopsis thaliana CSN4 Protein, N-His |
| 货号 | YZZ04401 |
| 别名 | COP9 signalosome complex subunit 4, AtS4, Signalosome subunit 4, Constitutive photomorphogenesis protein 8, Protein FUSCA 4, CSN4, COP8, FUS4, At5g42970, MBD2.17 |
| 表达系统 | E. coli |
| Accession号 | Q8L5U0 |
| 种属 | Arabidopsis thaliana (Mouse-ear cress) |
| 蛋白长度 | Asp2-Val397 |
| 预测分子量 | 46.06 kDa |
| 性质 | Recombinant |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 状态 | Lyophilized |
| 保存溶液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 说明 | For research use only. |

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文献和实验A Pipeline for 15N Metabolic Labeling and Phosphoproteome Analysis in Arabidopsis thaliana
experimental samples in a high-throughput, reproducible fashion with minimal technical variability. This chapter describes a pipeline used for labeling living Arabidopsis thaliana plants with nitrogen-15 (15 N) and how this can be used, in conjunction
基因组中最小的。每个单倍染色体组( n=5)的总长只有 7000万个碱基对,即只有小麦染色体组长的 1/80,这就使克隆它的有关基因相对说来比较容易。拟南芥是自花受粉植物,基因高度纯合,用理化因素处理突变率很高,容易获得各种代谢功能的缺陷型。例如用含杀草剂的培养基来筛选,一般获得抗杀草剂的突变率是 1/100000。由于有上述这些优点,所以拟南芥是进行遗传学研究的好材料,被科学家誉为“植物中的果蝇”。
ARABIDOPSIS THALIANA DNA ISOLATION PROCEDURE
Nuclei isolation modified from Hamilton, Kunsch, and Temperli (1972) Anal. Biochem. 49:48-57; further modified by Tom Guilfoyle, then N. Olszewski and Eric Richards. Procedure: (all steps at 0-4C unless indicated) Harvest 100 g
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