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- 详细信息
- 文献和实验
- 技术资料
- 库存:
199
- 英文名:
[2H2]-D-Tyrosine
- CAS号:
1202064-22-9;CAS:556-02-5
- 保质期:
收到货为准
- 供应商:
齐一生物科技(上海)有限公司
- 保存条件:
见详细包装
- 规格:
100mg
[2H2]-D-酪氨酸CAS号:1202064-22-9;CAS:556-02-5
中文名称:[2H2]-D-酪氨酸
英文名称:[2H2]-D-Tyrosine
CAS号:1202064-22-9;CAS:556-02-5
标准品货号:QYT-25803
标准品规格:100mg
产品结构式:详细询问客服:
[2H2]-D-酪氨酸CAS号:1202064-22-9;CAS:556-02-5
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文献和实验Procedures for In Vitro Production of Bovine Embryos
tubes (05-539-5)18 g 1 ½” needles (14-826-5D)50 ml sterile centrifuge tubes (05-538-67) 60 X 15 petri dishes (08757100B) (Falcon 351007) Absorbent paper (14-206-65)Beakers 10 ml (02-540C) 50 ml (02-540G) 400 ml (02-555
于500ml SOB培养液中,37℃恒温,250rpm振荡培养至OD600=0.5-0.6(约2.5-3hr)。 SOB液体培养基,115℃,10lb/in2灭菌15min A. bacto-Tryptone 10.00g B. bacto-Yeast Extract 2.50g C. NaCl 0.25g D. KCl 0.09g E. MgCl2•6H2O 1.02g F. ddH2O → 500.0ml
可查,根据此光吸收值可以较准确地计算蛋白质浓度。下式列出了蛋白质浓度与(a1%1cm)值(即蛋白质溶液浓度为1%,光径为1cm时的光吸收值)的关系。文献值a1%1cm,称为百分吸收系数或比吸收系数。 蛋白质浓度= (a280′10 )/ a1%1cm,280nm (mg/ml) (q 1%浓度?10mg/ml) 例:牛血清清蛋白: a1%1cm=6.3 (280nm) 溶菌酶: a1%1cm=22.8 (280nm) 若查不到待测蛋白质的a1%1cm值,则可选用一种与待测蛋白质的酪氨酸
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