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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期:
1 year
- 英文名:
Recombinant serotype O1 ctxB/Cholera Toxin Subunit B Protein, N-His-SUMO
- 库存:
100ug
- 供应商:
abinScience
- 规格:
100ug
| Expression system | E. coli |
| Accession | P01556 |
| Protein length | Thr22-Asn124 |
| Alternative Names | Cholera enterotoxin subunit B, Cholera enterotoxin B chain, Cholera enterotoxin gamma chain, Choleragenoid, ctxB, toxB, VC_1456 |
| Form | Lyophilized |
| Predicted molecular weight | 23.99 kDa |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Nature | Recombi\t |
| Species | serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) |
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文献和实验Shiga Toxin B-Subunit as a Tool to Study Retrograde Transport
Shiga toxin, produced by Shigella dysenteriae and Shiga toxin-producing Escherichia coli , is a bacterial protein toxin composed of two subunits, A and B (1 ). The A-subunit is the actual toxin whose ribosomal RNA N -glycosidase activity
Isolation of Membrane Rafts and Signaling Complexes
, and Tritoninsoluble floating fraction. They are enriched in cholesterol, often sphingomyelin and various gangliosides (GM1, GM2, and GM3). The ability of the B-subunit of cholera toxin to bind GM1 has been exploited to visualize membrane rafts by confocal microscopy
Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
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