In Vivo Grade Recombinant Huma

The recombinant human IgG1 Fc protein (hIgG1 Fc) includes the CH2 and CH3 domains of the heavy chain constant region. Most of the therapeutic antibody drugs and Fc fusion proteins carry the natural human IgG1 Fc region or engineered mutants. The recombinant human IgG1 Fc protein can be used as the isotype control of the hIgG1 antibodies and the hIgG1 Fc fusion proteins in various in vitro and in vivo experiments. Changes to the IgG Fc sequence including glycosylation sites have been used to modulate Fc functions. Even a single mutation may have both enhancement and abrogation effects of Fc binding. The following mutations have been introduced to alter the Fc region of an IgG1 antibody or an IgG1 Fc fusion protein to change its immunologic properties by Fc interaction with host proteins: hIgG1 Fc R214K (Polatuzumab) hIgG1 Fc R214K/N297A (Atezolizumab) hIgG1 Fc L234A/L235A (Risankizumab) hIgG1 Fc L234F/L235E/P331S (Durvalumab) hIgG1 Fc L235A/G237A (Vedolizumab) hIgG1 Fc D356E/L358M (Ocrelizumab) hIgG1 Fc F126L (Necitumumab) hIgG1 Fc D265A hIgG1 Fc F243L/R292P/Y300L/V305I/P396L hIgG1 Fc S239D/I332E hIgG1 Fc S239D/I332E/A330L hIgG1 Fc S298A/E333A/K334A hIgG1 Fc G236A/S239D/I332E hIgG1 Fc K326W/E333S hIgG1 Fc S267E/H268F/S324T hIgG1 Fc E345R/E430G/S440Y hIgG1 Fc N297A hIgG1 Fc N297Q hIgG1 Fc N297G hIgG1 Fc L235E hIgG1 Fc L234A/L235A hIgG1 Fc M252Y/S254T/T256E hIgG1 Fc M428L/N434S hIgG1 Fc S267E/L328F hIgG1 Fc N325S/L328F Recombinant natural and engineered human IgG1 Fc proteins, and recombinant human IgG1 isotype control antibody and mutants are available. Biotinylated hIgG1 Fc, and hIgG1 Fc with Avi-, His-, and Flag-tags are also available. Please contact us to ask for a quote for the engineered human IgG1 Fc proteins, biotinylated hIgG1 Fc, and hIgG1 Fc with Avi-, His-, and Flag-tags.