- ￥620 - 2200
- 善本生物
- 3471
- 2025年09月04日
- Western Blotting (WB)
- 鼠
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 浓度：
Recommended Dilutions：Western Blotting (WB):1:2,500-1:5,000
- 应用范围：
Western Blotting (WB)
- 宿主：
鼠
- 适应物种：
Human
- 保质期：
One year
- 克隆性：
Mouse mAb
- 保存条件：
Store at -20 °C for one year. Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
- 形态：
液体
- 亚型：
Mouse IgG1
- 免疫原：
Recombinant protein of human VIM
- 规格：
20ul/50ul/100ul
| 规格： | 20ul | 产品价格： | ￥620.0 |
|---|---|---|---|
| 规格： | 50ul | 产品价格： | ￥1200.0 |
| 规格： | 100ul | 产品价格： | ￥2200.0 |
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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