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- 详细信息
- 文献和实验
- 技术资料
- 浓度:
Recommended Dilutions:Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
- 应用范围:
Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
- 宿主:
兔
- 适应物种:
Human, Mouse, Rat
- 保质期:
One year
- 克隆性:
Rabbit mAb
- 保存条件:
Store at -20 °C for one year. Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
- 形态:
液体
- 亚型:
Rabbit IgG
- 免疫原:
A synthesized peptide derived from human Y14
- 规格:
20ul/50ul/100ul
| 规格: | 20ul | 产品价格: | ¥620.0 |
|---|---|---|---|
| 规格: | 50ul | 产品价格: | ¥1200.0 |
| 规格: | 100ul | 产品价格: | ¥2200.0 |
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
2c + ++ Sources of information: Akerstrom, B., Brodin, T., Reis, K., and Bjorck, L. 1985. Protein G: A powerful tool for binding and detection of monoclonal and polyclonal antibodies. J. Immunol. 135:2589-2592. Akerstrom, B. and Bjorck, L. 1986
monoclonal anti-HA 16B12 (MMS101R, BAbCo, used at 1:200 to 1:1000 dilution) mouse monoclonal anti-HA 12CA5 (12CA5, Boehringer, used at 1:200 dilution) rabbit polyclonal anti-HA (RS1010C, BAbCo, used at 1:200 dilution) Cy3-conjugated affinity
Using Biacore to Measure the Binding Kinetics of an Antibody‐Antigen Interaction
?protein interaction is described using an antibody and its antigen as an example. The affinity of the antibody for its antigen is determined by measuring the binding kinetics of the interaction. The protocols are divided into three major steps
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