NCI-H146细胞

NCI-H146细胞NCI-H146人小细胞肺癌细胞

Cell line name NCI-H146

Synonyms H146; H-146; NCIH146

Accession CVCL_1473

Resource Identification Initiative To cite this cell line use: NCI-H146 (RRID:CVCL_1473)

Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Population: Caucasian.

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Array-based CGH.

Omics: Deep exome analysis.

Omics: Deep proteome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: miRNA expression profiling.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Derived from site: Metastatic; Bone marrow; UBERON=UBERON_0002371.

Sequence variations

Mutation; HGNC; HGNC:7133; KMT2D; Simple; p.Glu2911Ter (c.8731G>T); Zygosity=Heterozygous (Cosmic-CLP=910899; DepMap=ACH-000506).

Mutation; HGNC; HGNC:7133; KMT2D; Simple; p.Glu4588Ter (c.13762G>T); Zygosity=Heterozygous (Cosmic-CLP=910899; DepMap=ACH-000506).

Mutation; HGNC; HGNC:9884; RB1; Simple; p.Gln850Ter (c.2548C>T); ClinVar=VCV000284733; Zygosity=Homozygous (Cosmic-CLP=910899; DepMap=ACH-000506).

Mutation; HGNC; HGNC:11998; TP53; Simple; p.Pro318Leufs*21 (c.953_971del19); Zygosity=Heterozygous (Cosmic-CLP=910899; DepMap=ACH-000506).

HLA typing Source: PubMed=25960936

Class I

HLA-A A*01:01,03:01

HLA-B B*14:02,14:02

HLA-C C*08:02,16:01

Class II

HLA-DQ DQB1*04:01,04:01

 

Source: PubMed=26589293

Class I

HLA-A A*01:01,03:01

HLA-B B*14:02,14:02

HLA-C C*08:02,16:01

Genome ancestry Source: PubMed=30894373

 

Origin % genome

African 0.37

Native American 0

East Asian, North 2.05

East Asian, South 0

South Asian 0.45

European, North 66.14

European, South 30.99

Disease Lung small cell carcinoma (NCIt: C4917)

Small cell lung cancer (ORDO: Orphanet_70573)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Sex of cell Male

Age at sampling 59Y

Category Cancer cell line

STR profile Source(s): ATCC=HTB-173; CLS=300182; Cosmic-CLP=910899; KCLB=30173; PubMed=24586258; PubMed=25877200

 

Markers:

Amelogenin X

CSF1PO 11 (ATCC=HTB-173; CLS=300182; Cosmic-CLP=910899; KCLB=30173; PubMed=25877200)

11,12 (PubMed=24586258)

D3S1358 11

D5S818 12

D7S820 9,10

D8S1179 12,14

D13S317 11,12

D16S539 11

D18S51 15,17

D21S11 30

FGA 23

Penta D 13

Penta E 12

TH01 6,9.3

TPOX 8,11

vWA 14,16

 

Run an STR similarity search on this cell line

Publications

PubMed=6108156

Gazdar A.F., Carney D.N., Russell E.K., Sims H.L., Baylin S.B., Bunn P.A. Jr., Guccion J.G., Minna J.D.

Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties.

Cancer Res. 40:3502-3507(1980)

 

PubMed=6270685; DOI=10.1073/pnas.78.7.4591; PMCID=PMC319839

Cuttitta F., Rosen S.T., Gazdar A.F., Minna J.D.

Monoclonal antibodies that demonstrate specificity for several types of human lung cancer.

Proc. Natl. Acad. Sci. U.S.A. 78:4591-4595(1981)

 

PubMed=6272398; DOI=10.1126/science.6272398

Moody T.W., Pert C.B., Gazdar A.F., Carney D.N., Minna J.D.

High levels of intracellular bombesin characterize human small-cell lung carcinoma.

Science 214:1246-1248(1981)

 

PubMed=6148444; DOI=10.1093/jnci/73.4.801

Morstyn G., Russo A., Carney D.N., Karawya E., Wilson S.H. Jr., Mitchell J.B.

Heterogeneity in the radiation survival curves and biochemical properties of human lung cancer cell lines.

J. Natl. Cancer Inst. 73:801-807(1984)

 

PubMed=6713399

Rosen S.T., Mulshine J.L., Cuttitta F., Fedorko J., Carney D.N., Gazdar A.F., Minna J.D.

Analysis of human small cell lung cancer differentiation antigens using a panel of rat monoclonal antibodies.

Cancer Res. 44:2052-2061(1984)

 

PubMed=2985257

Carney D.N., Gazdar A.F., Bepler G., Guccion J.G., Marangos P.J., Moody T.W., Zweig M.H., Minna J.D.

Establishment and identification of small cell lung cancer cell lines having classic and variant features.

Cancer Res. 45:2913-2923(1985)

 

PubMed=2986243; DOI=10.1007/978-3-642-82372-5_4

Whang-Peng J., Lee E.C.

Cytogenetics of human small cell lung cancer.

Recent Results Cancer Res. 97:37-46(1985)

 

PubMed=3335022

Alley M.C., Scudiero D.A., Monks A., Hursey M.L., Czerwinski M.J., Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H., Boyd M.R.

Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.

Cancer Res. 48:589-601(1988)

 

PubMed=2388294; DOI=10.1093/jnci/82.17.1420

McLemore T.L., Litterst C.L., Coudert B.P., Liu M.C., Hubbard W.C., Adelberg S., Czerwinski M.J., McMahon N.A., Eggleston J.C., Boyd M.R.

Metabolic activation of 4-ipomeanol in human lung, primary pulmonary carcinomas, and established human pulmonary carcinoma cell lines.

J. Natl. Cancer Inst. 82:1420-1426(1990)

 

PubMed=1320893; DOI=10.1159/000163712

Verbeeck M.A.E., Elands J.P.M., de Leij L.F.M.H., Buys C.H.C.M., Carney D.N., Bepler G., Roebroek A.J.M., van de Ven W.J.M., Burbach J.P.H.

Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines.

Pathobiology 60:136-142(1992)

 

PubMed=1563005

Giaccone G., Battey J., Gazdar A.F., Oie H.K., Draoui M., Moody T.W.

Neuromedin B is present in lung cancer cell lines.

Cancer Res. 52:2732s-2736s(1992)

 

PubMed=8626706; DOI=10.1074/jbc.271.19.11477

Quinn K.A., Treston A.M., Unsworth E.J., Miller M.-J., Vos M., Grimley C., Battey J., Mulshine J.L., Cuttitta F.

Insulin-like growth factor expression in human cancer cell lines.

J. Biol. Chem. 271:11477-11483(1996)

 

PubMed=8806092; DOI=10.1002/jcb.240630505

Phelps R.M., Johnson B.E., Ihde D.C., Gazdar A.F., Carbone D.P., McClintock P.R., Linnoila R.I., Matthews M.J., Bunn P.A. Jr., Carney D.N., Minna J.D., Mulshine J.L.

NCI-Navy Medical Oncology Branch cell line data base.

J. Cell. Biochem. Suppl. 24:32-91(1996)