NCI-H196细胞

NCI-H196细胞NCI-H196人小细胞肺癌细胞

Cell line name NCI-H196

Synonyms H196; H-196; NCIH196

Accession CVCL_1509

Resource Identification Initiative To cite this cell line use: NCI-H196 (RRID:CVCL_1509)

Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Population: Caucasian.

Doubling time: 64 hours (PubMed=25984343).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Deep exome analysis.

Omics: Deep proteome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: shRNA library screening.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Caution: Originally classified as originating from a lung small cell carcinoma, but is reclassified as a lung sarcomatoid carcinoma based on a number of criteria (PubMed=38180245).

Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.

Sequence variations

Mutation; HGNC; HGNC:9884; RB1; Simple; p.Gln383Ter (c.1147C>T); ClinVar=VCV000428664; Zygosity=Homozygous (DepMap=ACH-000752).

Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg175His (c.524G>A); ClinVar=VCV000012374; Zygosity=Homozygous (DepMap=ACH-000752).

HLA typing Source: PubMed=25960936

Class I

HLA-A A*29:01,33:03

HLA-B B*07:05,50:01

HLA-C C*06:02,15:05

Class II

HLA-DR DRB1*10:01,15:25

 

Source: PubMed=26589293

Class I

HLA-A A*29:01,33:03

HLA-B B*07:05,50:01

HLA-C C*06:02,15:05

Genome ancestry Source: PubMed=30894373

 

Origin % genome

African 0

Native American 0.1

East Asian, North 0

East Asian, South 1.64

South Asian 3.75

European, North 50.92

European, South 43.59

Disease Lung sarcomatoid carcinoma (NCIt: C45540)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Sex of cell Male

Age at sampling 68Y

Category Cancer cell line

STR profile Source(s): ATCC=CRL-5823; CLS=300390; Cosmic-CLP=1240186; PubMed=25877200

 

Markers:

Amelogenin X,Y

CSF1PO 10

D2S1338 17,20

D3S1358 15

D5S818 12

D6S1043 13

D7S820 10,11

D8S1179 13,15

D12S391 19

D13S317 9

D16S539 11

D18S51 17,19

D19S433 14

FGA 22,23

Penta D 10

Penta E 8,12

TH01 6

TPOX 11

vWA 19

 

Run an STR similarity search on this cell line

Publications

PubMed=2985257

Carney D.N., Gazdar A.F., Bepler G., Guccion J.G., Marangos P.J., Moody T.W., Zweig M.H., Minna J.D.

Establishment and identification of small cell lung cancer cell lines having classic and variant features.

Cancer Res. 45:2913-2923(1985)

 

PubMed=2986243; DOI=10.1007/978-3-642-82372-5_4

Whang-Peng J., Lee E.C.

Cytogenetics of human small cell lung cancer.

Recent Results Cancer Res. 97:37-46(1985)

 

PubMed=8806092; DOI=10.1002/jcb.240630505

Phelps R.M., Johnson B.E., Ihde D.C., Gazdar A.F., Carbone D.P., McClintock P.R., Linnoila R.I., Matthews M.J., Bunn P.A. Jr., Carney D.N., Minna J.D., Mulshine J.L.

NCI-Navy Medical Oncology Branch cell line data base.

J. Cell. Biochem. Suppl. 24:32-91(1996)

 

PubMed=8806103; DOI=10.1002/jcb.240630516

Johnson B.E., Russell E., Simmons A.M., Phelps R.M., Steinberg S.M., Ihde D.C., Gazdar A.F.

MYC family DNA amplification in 126 tumor cell lines from patients with small cell lung cancer.

J. Cell. Biochem. Suppl. 24:210-217(1996)

 

PubMed=11030152; DOI=10.1038/sj.onc.1203815

Modi S., Kubo A., Oie H.K., Coxon A.B., Rehmatulla A., Kaye F.J.

Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.

Oncogene 19:4632-4639(2000)

 

PubMed=17426248; DOI=10.1158/1541-7786.MCR-06-0367

Olejniczak E.T., Van Sant C., Anderson M.G., Wang G., Tahir S.K., Sauter G., Lesniewski R., Semizarov D.

Integrative genomic analysis of small-cell lung carcinoma reveals correlates of sensitivity to bcl-2 antagonists and uncovers novel chromosomal gains.

Mol. Cancer Res. 5:331-339(2007)

 

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662

Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Cancer Res. 70:2158-2164(2010)