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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温
- 保质期:
一年
- 英文名:
Dowex<<@>> 1X8 chloride form
- 库存:
100
- 供应商:
科淼生物
- CAS号:
69011-19-4
- 规格:
100g
产品中文名称:Dowex@ 1×8 氯化物形式, chloride form
产品英文名称:Dowex<<@>> 1X8 chloride form
CAS号:69011-19-4
纯度级别:100-200 mesh
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文献和实验Competent cells, calcium chloride method, E. coli, description
(4000-5000 rpm for 5 min or 5000 x g for 5 min) at 4 C Keep the cells ice cold in all further steps. Resuspend the cells in 1/2 culture volume of 0.1 M ice-cold CaCl2. Hold on ice for minimum of 30 min, prefably 1 - 2 h
is an indirect expression of the monoclonal antibody amount in the sample.Methods1 Obtain a 96-well plate and rinse 3x with PBS.2 Add 50 µl of a 1 mg/ml of antigen (venom, serum, or fraction), incubate for 30 min at 37°C.3 Rinse with PBS 3x.4 Add 5% powdered milk
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
reaction buffer can be stored for at least one year at room temperature in foil-covered container. X-gal stock(40X) 40 mg/ml X-gal in dimethylformamide Store at -200 C in foil covered glass container. Rinse many times in PBS
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