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从原料到标记,打造属于中国自己的流式抗体品牌abinScience 体内级抗体,年度锁价,低至 1200五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2a, kappa
- 形态:
Liquid
- 保存条件:
0.01M PBS, pH 7.4.
- 克隆性:
Monoclonal
- 保质期:
1 year
- 抗原来源:
Mouse
- 目录编号:
HB065013
- 级别:
科研级别
- 库存:
999
- 供应商:
abinscience
- 浓度:
1mg/ml
- 抗体英文名:
Anti-nNOS/NOS1 Antibody (L121/25)
- 抗体名:
Anti-nNOS/NOS1 Antibody (L121/25)
- 规格:
50ug

| Product name | Anti-nNOS/NOS1 Antibody (L121/25) |
|---|---|
| Catalog No. | HB065013 |
| Host species | Mouse |
| Specificity | \ |
| Conjugation | \ |
| Species reactivity | \ |
| Immunogen | \ |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG2a, kappa |
| Applications | IHC, WB |
| Target | Constitutive NOS, Neuronal NOS, nNOS, N-NOS, bNOS, NOS type I, NC-NOS, Peptidyl-cysteine S-nitrosylase NOS1, NOS1, Nitric oxide synthase, brain |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | \ |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Background | \ |
| Alternative Names | \ |
| Clone ID | \ |
| Species | \ |
| Note | For research use only. |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验monoclonal antibody production fusion
), 3 L, 0.22 (or 0.45) µm-filtered, store at -20ѓC = Asp-Arg x100, 1 L + Gluta x 50, 2 L + mercaptoethanol, 350 µl Asp-Arg x100, 1 L, pH 6.0 = L-Asparagine, 3.6 g (0.24 mM) + L-Arginine (0.55 mM), 11.6 g + HCl, 0.5N, 100 ml -> heat to 37ѓC
糖,建议进行 Pre-clear;如果使用磁珠,此步可忽略 抗 X 抗体的轻链(25 kD)和重链(50 kD) 更侧重于如何在实验上进行优化(见下文) *注意:总蛋白上样量过多也会造成 非特异性结合,建议上样量为 500 ug-1 mg。上样之前一定要先用 BCA 定个量!定个量!定个量! pre-clear:上样前先用裂解液过一遍柱子,减少基质本身对蛋白的吸附 Output:在某些 co-IP 实验中,实验人员会把IP后的上清分别进行诱饵蛋白 X 和靶蛋白 Y 的 WB 检测
扫描仪中,都采用机械式的二维X,Y线性扫描技术实现,即X,Y方向都采用直线驱动器和直线导轨实现往复运动。此类装置,由于驱动系统的频率限制,驱动器的扫描惯性大,使得扫描效率低,分析时间相当长;并且往复行程长,对直线导轨的精度要求相当高。二、光机结合的二维扫描系统为同样实现生物芯片的二维扫描,我们的实验装置设计如图2,采用了振镜和大数值孔径的远心f-è物镜相结合实现X方向扫描,Y方向的运动仍采用直线驱动器和直线导轨实现。 系统中,对于f-è物镜,满足x=2fè(è为振镜的摆动角度,f为物镜焦距)的线性
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