- 询价
- abinscience
- France
- HB927117
- 2026年03月03日
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1, kappa
- 形态：
Liquid
- 保存条件：
0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.
- 克隆性：
Monoclonal
- 标记物：
FITC
- 适应物种：
Human
- 保质期：
1 year
- 抗原来源：
Mouse
- 目录编号：
HB927117
- 级别：
科研级别
- 库存：
999
- 供应商：
abinscience
- 浓度：
1mg/ml
- 抗体英文名：
Anti-Human CD140a/PDGFRA Antibody (SAA0056), FITC
- 抗体名：
Anti-Human CD140a/PDGFRA Antibody (SAA0056), FITC
- 规格：
100ug
| Product name | Anti-Human CD140a/PDGFRA Antibody (SAA0056), FITC |
|---|---|
| Catalog No. | HB927117 |
| Host species | Mouse |
| Specificity | \ |
| Conjugation | FITC |
| Species reactivity | Human |
| Immunogen | \ |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. |
| Purity | \ |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Applications | FCM |
| Target | PDGFR2, Platelet-derived growth factor receptor alpha, RHEPDGFRA, Alpha platelet-derived growth factor receptor, PDGFR-2, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, PDGF-R-alpha, Alpha-type platelet-derived growth factor receptor, CD140a antigen, CD140 antigen-like family member A, PDGFRA, PDGFR-alpha, CD140a |
| Purification | \ |
| Accession | P16234 |
| Stability and Storage | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| Background | \ |
| Alternative Names | \ |
| Clone ID | SAA0056 |
| Species | \ |
| Note | For research use only. |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验【摘要】目的探讨嵌合抗CD20抗体Fab’片段的抗肿瘤机制。方法应用MTT法检测Fab’片段对Raji细胞生长的抑制作用，用Annexin V-FITC和PI测定Fab’片段对Raji细胞凋亡的诱导作用，用RT-PCR和Western blot检测Raji细胞中bcl-2表达的变化。结果嵌合抗CD20Fab’片段对Raji细胞具有明显的抑制作用IC50值为24.2 µg/ml，并呈剂量依赖性；嵌合抗CD20Fab’片段能诱导Raji细胞的凋亡，并降低Raji细胞中bcl-2基因及其蛋白的表达
，用含有10%热灭活胎牛血清的RPMI1640培养基于5% CO2，37C条件下培养。抗CD20单克隆抗体HI47由我所免疫室提供。辣根过氧化酶标记羊抗人IgG，FITC标记的羊抗鼠IgG购于北京中山生物工程公司。2、抗CD20单克隆抗体轻、重链基因的克隆利用RT-PCR法，采用抗体通用的兼并性引物，从HI47杂交瘤细胞特异性地分别扩增抗体轻链、重链可变区基因（VL和VH）后，利用编码短肽（G4S）3的连接链DNA片段，经overlap PCR，将VH和VL基因连接构成ScFv基因（约750bp
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