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- 文献和实验
- 技术资料
- 保存条件:
常温
- 保质期:
一年
- 英文名:
Oestradiol 17-heptanoate
- 库存:
100
- 供应商:
科淼生物
- CAS号:
4956-37-0
- 规格:
1g
产品中文名称:庚酸雌二醇
产品英文名称:Oestradiol 17-heptanoate
CAS号:4956-37-0
纯度级别:98%
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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of aggregation plate Materials: Dissecting microscope; Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3); 1 ml syringe with 26 G 1/2 needle of KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);
The ribonuclease protection assay (RPA)
by gentle flicking and quick spin in a microfuge. Incubate at 37°C for 30 minutes. 3. Add the following reagents (in order) to each 1.5 ml Eppendorf tube: 26 µl 20 mM EDTA 25 µl Tris-saturated phenol 25 µl chloroform:isoamyl alcohol (50
Steady State ATPase Assays Coupled Enzyme System
DDW 10 mM Mg・ATP 30 mM PEP 6 mM NADH (Make fresh in 10 mM Tris-OAc pH 7.5, 4.26 mg/mL = 6 mM) PK/LDH (Sigma #P-0294, PK + LDH
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