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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温
- 保质期:
一年
- 英文名:
Boc-D-Gly(allyl)-OH.DCHA
- 库存:
100
- 供应商:
科淼生物
- CAS号:
221352-64-3
- 规格:
1g
产品中文名称:Boc-D-Gly(allyl)-OH.DCHA
产品英文名称:Boc-D-Gly(allyl)-OH.DCHA
CAS号:221352-64-3
纯度级别:97%
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文献和实验Amino-allyl Reverse Transcription
with the amino-allyl dye coupling procedure all Tris must be removed from the reaction to prevent the monofunctional NHS-ester Cye dyes from coupling to free amine groups in solution. Fill one Microcon 30 concentrator with 450 ul water. Add neutralized
wash buffer. Spin. Discard flow-through. 5. Elute in amber 1.5 mL tube: Add 50 µL H2 O. Incubate 1 min. Spin 1 min. Repeat elution a 2nd time into the same tube. (Optional: save aliquot to measure 32P counts) 7. Dry in speed-vac. D.
Revised amino-allyl labeling protocol
and the Howard Hughes Medical Institute http://cmgm.stanford.edu/pbrown/protocols/amino-allyl.htm A. RT Reaction 1. To anneal primer, mix 1-2 m g mRNA with 5 ug of anchored oligo-dT [(dT)20 -VN] (Operon, HPLC purified) in a total volume of 18 m L
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