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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温
- 保质期:
一年
- 英文名:
<small>DL</small>-Alanyl-<small>L</small>-alanine
- 库存:
100
- 供应商:
科淼生物
- CAS号:
59247-16-4
- 规格:
100mg
产品中文名称:DL-丙氨酰-L-丙氨酸
产品英文名称:DL-Alanyl-L-alanine
CAS号:59247-16-4
纯度级别:>98.0%(HPLC)(T)
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文献和实验Single Primer ("Semi-Random") P
to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL Cycle as follows: 30"x94°C 20 cycles 0"x94°C 0"x55°C 1'x72° S=9 30 cycles 0"x94°C 0"x40°C 1'x72° S=6 30 cycles 0"x94°C 0"x55°C 1'x72° S=9 Clean up: Add 1 µL ExoI
Single tube confirmation PCR protocol
(200mg/l). - Incubate at 30°C for two to three days. *This step reduces background by eliminating aborted transformants. 2. Zymolyase treatment - Transfer a small portion of a well-isolated colony (~1 mm) into 50 µl solution
Alternate Sporulation Method for BY4743
pre-sporulation plate for one more day at 30°C. 2. Transfer to liquid sporulation medium by resuspending a small amount of cells int 2mls of supplemented liquid sporulation medium. Don't put in too many cells! 3. Incubate on a roller
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