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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20度
- 保质期:
一年
- 英文名:
Recombinant Mouse Acidic Fibroblast Growth Factor (Mouse aFGF)
- 库存:
现货
- 供应商:
上海魔兜儿
- 规格:
10ug/100ug/500ug
| 规格: | 10ug | 产品价格: | ¥986.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥3586.0 |
| 规格: | 500ug | 产品价格: | ¥8172.0 |
产品参数
|
别名(Synonyms) | Fibroblast growth factor 1;FGF-1;Acidic fibroblast growth factor;HBGF-1 |
|
Accession | P61148 |
|
GeneID | 14164 |
|
表达系统(Source) | E.coli-derived mouse Acidic Fibroblast Growth Factor protein, Phe16-Asp155 |
|
分子量大小(Molecular Weight) | Approximately 15.8 kDa. |
|
氨基酸序列(AA Sequence) | FNLPLGNYKK PKLLYCSNGG HFLRILPDGT VDGTRDRSDQ HIQLQLSAES AGEVYIKGTE TGQYLAMDTE GLLYGSQTPN EECLFLERLE ENHYNTYTSK KHAEKNWFVG LKKNGSCKRG PRTHYGQKAI LFLPLPVSSD |
|
标签(Tag) |
None |
|
外观(Physical Appearance) | Sterile Filtered White lyophilized (freeze-dried) powder. |
|
纯度(Purity) | > 96 % by SDS-PAGE and HPLC analyses. |
|
生物活性(Biological Activity) | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg in the presence of 10 μg/ml of heparin. |
|
内毒素(Endotoxin) | < 1.0 EU per 1μg of the protein by the LAL method. |
|
制剂(Formulation) | Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
|
复溶(Reconstitution) | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
储存条件
产品用冰袋装运,可在-20℃至-80℃下保存1年。
建议第一次使用时将蛋白质分成较小的量,并避免反复冻融循环。
注意事项
1.避免反复冻融循环。
2.为了您的安全和健康,请穿实验服,戴一次性手套进行操作。
3.仅供研究使用!
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文献和实验Analysis of Growth Properties and Cell Cycle Regulation Using Mouse Embryonic Fibroblast Cells
as an invaluable tool to study altered growth properties of cells and identify regulatory molecules involved in neoplastic transformation. In this chapter, protocols for isolation of mouse embryonic fibroblast cells from midgestation mouse embryos
ABSTRACT Fibroblast growth factor (FGF) signaling plays a signifigant role in embryonic development. Particularyl, FGFs are implicated in mediating epithelial-mesenchymal interactions by means of regulating cell proliferation
指在组织培养中,除了氨基酸、维生素、葡萄糖以及无机盐等正常成分之外,其可以代替培养基血清高分子物质的而促进细胞生长的物质。与发育因子和增殖因子为同义词,但因在组织培养中是以细胞增殖为直接目的,所以称增殖因素较确切。生长因子多为广义的肽激素,有胰岛素、表皮生长因素(EGF)、成纤细胞生长因素(fibroblast gfowth factor,FGF)、血小板来源增殖因素(platelet-derivedgrowth factor,PDGF)以及生长
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