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- 技术资料
- 保存条件:
常温
- 保质期:
一年
- 英文名:
methanesulfonate salt
- 库存:
100
- 供应商:
科淼生物
- CAS号:
105737-62-0
- 规格:
5mg
产品中文名称:CGP-20712A
产品英文名称:methanesulfonate salt
CAS号:105737-62-0
纯度级别:≥98%(HPLC)
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文献和实验浅析染色质免疫沉淀(ChIP)技术在 DNA 与蛋白质相互作用研究中的重要性
, B.E., et al., A bivalent chromatin structure marks key developmental genes in embryonic stem cells. Cell, 2006. 125(2): p. 315-26. [19] Topp, C.N., C.X. Zhong, and R.K. Dawe, Centromere-encoded RNAs are integral components of the maize kinetochore
In vitro transcription reaction
X Denhardts 2 10 X 10 mg/ml (3 min fresh boiled )salmon sperm DNA 0.1 100 mg/ml 20% SDS 0.1 0.2% Final Volume 10 1- Pre-heat buffer to 62 oC add salmon sperm. Pre hybridize at 62 oC for 4 h. 2- Add RNA probe to final conc. of 5X10 6cpm/ml to the pre
In vitro transcription reaction
% 20X SSPE 2.5 5X 50X Denhardts 2 10 X 10 mg/ml (3 min fresh boiled )salmon sperm DNA 0.1 100 mg/ml 20% SDS 0.1 0.2% Final Volume 10 1- Pre-heat buffer to 62 oC add salmon sperm. Pre hybridize at 62 oC for 4 h. 2- Add RNA probe to final conc
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