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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温
- 保质期:
一年
- 英文名:
Eicosapentaenoyl Chloride
- 库存:
100
- 供应商:
科淼生物
- CAS号:
98770-65-1
- 规格:
5mg
产品中文名称:Eicosapentaenoyl Chloride
产品英文名称:Eicosapentaenoyl Chloride
CAS号:98770-65-1
纯度级别:98%
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文献和实验Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction
Procedure 1. Grind 2 to 5 g of frozen leaves to a very fine powder with a liquid nitrogen-cooled mortar and pestle. 2. Add 25 ml of CTAB Buffer and transfer to a 50 ml tube. 3. Incubate at 65°C for 20 min with occasional vigorous
in cesium chloride-ethidium bromide gradients (1). Briefly, cells containing the desired plasmid or cosmid are harvested by centrifugation, incubated in a lysozyme buffer, and treated with alkaline detergent. Detergent solubilized proteins and membranes
Plasmid DNA Isolation, Alkaline Lysis(碱裂解法分离质粒DNA)
. 2. Maintain at 65℃ for 15 min. and then centrifuge for 15 min at 12,000 x g . 3. Save supernatant and precipitate by adding 2 vol. of cold ethanol, etc. 4. Collect pellet and reprecipitate in TE-ammonium acetate as above.
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