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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2a, kappa
- 形态:
Liquid
- 保存条件:
0.01M PBS, pH 7.4.
- 克隆性:
Monoclonal
- 适应物种:
Escherichia coli
- 保质期:
1 year
- 抗原来源:
Mouse
- 目录编号:
JN827013
- 级别:
科研级别
- 库存:
999
- 供应商:
abinscience
- 浓度:
1mg/ml
- 抗体英文名:
Anti-BRIL tag Antibody (SAA2112)
- 抗体名:
Anti-BRIL tag Antibody (SAA2112)
- 规格:
100ug

| Product name | Anti-BRIL tag Antibody (SAA2112) |
|---|---|
| Catalog No. | JN827013 |
| Host species | Mouse |
| Specificity | \ |
| Conjugation | \ |
| Species reactivity | Escherichia coli |
| Immunogen | \ |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Purity | \ |
| Clonality | Monoclonal |
| Isotype | IgG2a, kappa |
| Applications | ELISA |
| Target | Soluble cytochrome b562, Cytochrome b-562, cybC |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | \ |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Background | \ |
| Alternative Names | \ |
| Clone ID | SAA2112 |
| Species | \ |
| Note | For research use only. |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验DNA聚合酶,限制性内切酶mluI,nheI,sphI,notI均购自GIBCOBRL公司,抗CD3单克隆抗体HIT3a由本所协和科技公司提供,抗Pgp单克隆抗体PHMA02由本室制备。鼠抗E-tag抗体,HRP标记的鼠抗E-tag抗体及抗E-tag亲和层析柱均购自Pharmacia公司。FITC标记的羊抗鼠IgG多抗购自北京中山生物工程公司。其它均为国产分析纯生化试剂。(3)PCR引物P1 5’ CTA GCG CAC GCG TAC GCT GAC ATC GAG CTC ACT CAG TCT
【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体?
对照,2道为1:1000倍稀释我们先看一下EarthOx的单克隆GFP抗体——Anti-GFP Tag Monoclonal Antibody [GF28R](货号#E022030),其免疫原是KLH偶联的水母GFP蛋白的N末端多肽,由于EGFP、YFP、EYFP、CFP得N末端序列与GFP几乎完全一致,因此从免疫原上来说,GFP与EGFP、YFP、EYFP、CFP没有区别。更重要的是,EarthOx对于该单克隆抗体还进行了明确的验证试验,试验也表明该Anti-GFP Tag Monoclonal
培养箱中培养。 2、实验结果 图:1×10^6/孔细胞,5μg anti-mouse CD3与CD28刺激3天后细胞形成明显克隆团 CD3/CD28 Streptamer® 多聚体法 1、实验原理 使用低亲和力的抗 CD3 和抗CD28的抗体 Fab 片段(非传统全长抗体),结合上 Twin-Strep-tag 亲和标签,形成 CD3 Fab-Strep 和 CD28 Fab-Strep。再结合可溶性蛋白多聚体 Strep-Tactin®,在亲和力作用下,即可与 TCR 和 CD28 共刺激
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