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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1, kappa
- 形态:
Liquid
- 保存条件:
0.01M PBS, pH 7.4.
- 克隆性:
Monoclonal
- 适应物种:
MPXV/MPOX
- 保质期:
1 year
- 抗原来源:
Human
- 目录编号:
VK680023
- 级别:
科研级别
- 库存:
999
- 供应商:
abinscience
- 浓度:
1mg/ml
- 抗体英文名:
Anti-MPXV E8L Antibody (SAA0285)
- 抗体名:
Anti-MPXV E8L Antibody (SAA0285)
- 规格:
50ug

| Product name | Anti-MPXV E8L Antibody (SAA0285) |
|---|---|
| Catalog No. | VK680023 |
| Host species | Human |
| Specificity | \ |
| Conjugation | \ |
| Species reactivity | MPXV/MPOX |
| Immunogen | \ |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Applications | ELISA, Neutralization, WB |
| Target | E8L, Cell surface-binding protein, Carbonic anhydrase homolog |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | NP_536532.1 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Background | \ |
| Alternative Names | \ |
| Clone ID | SAA0285 |
| Species | \ |
| Note | For research use only. |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验DNA聚合酶,限制性内切酶mluI,nheI,sphI,notI均购自GIBCOBRL公司,抗CD3单克隆抗体HIT3a由本所协和科技公司提供,抗Pgp单克隆抗体PHMA02由本室制备。鼠抗E-tag抗体,HRP标记的鼠抗E-tag抗体及抗E-tag亲和层析柱均购自Pharmacia公司。FITC标记的羊抗鼠IgG多抗购自北京中山生物工程公司。其它均为国产分析纯生化试剂。(3)PCR引物P1 5’ CTA GCG CAC GCG TAC GCT GAC ATC GAG CTC ACT CAG TCT
挑选14个克隆进行筛选,结果发现14个克隆均能与靶抗原有反应,其中以4(E4F4)、5(E5F5)、6(G4)、7(G5)、11(G9)克隆颜色较深,挑取6号克隆,利用APAAP法进一步检测其结合活性。结果显示,克隆6的表达产物能和Daudi细胞特异结合。克隆6的测序结果和氨基酸序列分析表明该基因序列完全符合PDB库中抗体所具有的若干保守的框架氨基酸的特点,该序列是一抗体基因序列。2、抗CD20嵌合抗体Fab和F(ab)2的表达与纯化SDS-PAGE电泳结果表明Protein G可有效纯化Fab
【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体?
对照,2道为1:1000倍稀释我们先看一下EarthOx的单克隆GFP抗体——Anti-GFP Tag Monoclonal Antibody [GF28R](货号#E022030),其免疫原是KLH偶联的水母GFP蛋白的N末端多肽,由于EGFP、YFP、EYFP、CFP得N末端序列与GFP几乎完全一致,因此从免疫原上来说,GFP与EGFP、YFP、EYFP、CFP没有区别。更重要的是,EarthOx对于该单克隆抗体还进行了明确的验证试验,试验也表明该Anti-GFP Tag Monoclonal
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