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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.
- 克隆性:
Polyclonal
- 标记物:
PE
- 保质期:
1 year
- 抗原来源:
Goat
- 目录编号:
ZF690127
- 级别:
科研级别
- 库存:
999
- 供应商:
abinscience
- 浓度:
1mg/ml
- 抗体英文名:
Normal Goat IgG Isotype Control Antibody, PE
- 抗体名:
Normal Goat IgG Isotype Control Antibody, PE
- 规格:
100ug

| Product name | Normal Goat IgG Isotype Control Antibody, PE |
|---|---|
| Catalog No. | ZF690127 |
| Host species | Goat |
| Specificity | \ |
| Conjugation | PE |
| Species reactivity | \ |
| Immunogen | \ |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. |
| Purity | \ |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | FCM, Isotype Control |
| Target | \ |
| Purification | \ |
| Accession | \ |
| Stability and Storage | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| Background | \ |
| Alternative Names | \ |
| Clone ID | \ |
| Species | \ |
| Note | For research use only. |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验#01 什么是同型对照? 同型对照(Isotype Control),是指与使用的流式抗体具有相同种属来源、亚型、荧光标记物、使用剂量和浓度,但对目标靶点无特异性结合的抗体。同型对照通常用来消除抗体与抗原的非特异性结合造成的背景染色。 #02 为什么要做同型对照? 流式抗体与靶标的结合,是通过抗体的 Fab 端与靶标 Marker 进行特异性结合而完成的。白细胞(除T细胞外)表面大多表达 CD16、CD32、CD64 等 Fc 受体,这些受体可与各种抗体的 Fc 端非特异性结合,出现非特异
和相同的免疫球蛋白及亚型的免疫球蛋白,用于消除由于抗体非特异性与细胞结合而产生的背景染色。同型对照是真正意思上的阴性对照,它不但可以用来设定流式细胞仪的电压,而且还可以帮忙省去昂贵与繁琐的重组细胞因子竞争封闭步骤。在流式细胞仪上样前,染色方式如下: 样本管:一抗+样本 同型对照管:同型对照+样本 2 、如何选择同型对照? 样本管:纯化的CDw25+PE标记的抗Mouse IgG
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
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