- 询价
- abinscience
- France
- HY057337
- 2025年07月13日
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2a, kappa
- 形态：
Liquid
- 保存条件：
0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.
- 克隆性：
Monoclonal
- 标记物：
APC
- 适应物种：
Human
- 保质期：
1 year
- 抗原来源：
Mouse
- 目录编号：
HY057337
- 级别：
科研级别
- 库存：
999
- 供应商：
abinscience
- 浓度：
1mg/ml
- 抗体英文名：
Anti-Human CD3E Antibody (OKT3), APC
- 抗体名：
Anti-Human CD3E Antibody (OKT3), APC
- 规格：
100ug
| Product name | Anti-Human CD3E Antibody (OKT3), APC |
|---|---|
| Catalog No. | HY057337 |
| Host species | Mouse |
| Specificity | \ |
| Conjugation | APC |
| Species reactivity | Human |
| Immunogen | \ |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. |
| Purity | \ |
| Clonality | Monoclonal |
| Isotype | IgG2a, kappa |
| Applications | FCM |
| Target | T3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T-cell surface glycoprotein CD3 epsilon chain |
| Purification | \ |
| Accession | P07766 |
| Stability and Storage | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| Background | \ |
| Alternative Names | \ |
| Clone ID | \ |
| Species | \ |
| Note | For research use only. |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验Human PBMCs can be activated in vitro by soluble anti-human CD3 antibodies. We have performed titration studies with these antibodies and established the following protocol for stimulation of PBMC. Materials 1X sterile PBS Anti
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
a 5-10 µg/ml solution of anti-CD3e (145-2C11) in sterile PBS. Calculate the number of wells required for each experimental condition and consider triplicate samples for each condition. For example, to coat one-half plate (48 wells) 2.6 ml of antibody
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