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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2, kappa
- 形态:
Liquid
- 保存条件:
0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.
- 克隆性:
Monoclonal
- 标记物:
FITC
- 适应物种:
Human
- 保质期:
1 year
- 抗原来源:
Human
- 目录编号:
HF564117
- 级别:
科研级别
- 库存:
999
- 供应商:
abinscience
- 浓度:
1mg/ml
- 抗体英文名:
Anti-Human CD275/ICOSLG Antibody (SAA0089), FITC
- 抗体名:
Anti-Human CD275/ICOSLG Antibody (SAA0089), FITC
- 规格:
100ug

| Product name | Anti-Human CD275/ICOSLG Antibody (SAA0089), FITC |
|---|---|
| Catalog No. | HF564117 |
| Host species | Human |
| Specificity | \ |
| Conjugation | FITC |
| Species reactivity | Human |
| Immunogen | \ |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. |
| Purity | \ |
| Clonality | Monoclonal |
| Isotype | IgG2, kappa |
| Applications | FCM |
| Target | B7RP1, B7-related protein 1, CD275, ICOSL, ICOSLG, KIAA0653, B7 homolog 2, ICOS ligand, B7RP-1, B7H2, B7-H2, B7-like protein Gl50 |
| Purification | \ |
| Accession | O75144 |
| Stability and Storage | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| Background | \ |
| Alternative Names | \ |
| Clone ID | SAA0089 |
| Species | \ |
| Note | For research use only. |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验Dual- and Triple-Color Fluorospot
labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml
【摘要】目的探讨嵌合抗CD20抗体Fab’片段的抗肿瘤机制。方法应用MTT法检测Fab’片段对Raji细胞生长的抑制作用,用Annexin V-FITC和PI测定Fab’片段对Raji细胞凋亡的诱导作用,用RT-PCR和Western blot检测Raji细胞中bcl-2表达的变化。结果嵌合抗CD20Fab’片段对Raji细胞具有明显的抑制作用IC50值为24.2 µg/ml,并呈剂量依赖性;嵌合抗CD20Fab’片段能诱导Raji细胞的凋亡,并降低Raji细胞中bcl-2基因及其蛋白的表达
的注意事项: ①在用间接法染色时,染色步骤依次为第一抗体→清洗→第二抗体→清洗→红细胞溶解。为了实验的方便,将第二抗体也配成每次实验用10μl。 ②双色法:双色法多用于直接染色法。将分别用FITC和PE标记的抗体各10μl置入同一个含有约1×10 6 /0.2ml的试管内,30min,4℃避光。然后清洗、固定。 目前不少制备单克隆抗体的公司已将比值有意义的两种抗体,分别用红、绿荧光染料标记后,制成一种试剂。如CD 2 —FITC/CD 20 —PE;CD 4 -FITC
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