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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
0.01M PBS, pH 7.4, 0.09% Sodium azide.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
1 year
- 抗原来源:
Human
- 目录编号:
HP481207
- 级别:
科研级别
- 库存:
999
- 供应商:
abinscience
- 浓度:
1mg/ml
- 抗体英文名:
Anti-sLea/CA19-9 Antibody (7E3#)
- 抗体名:
Anti-sLea/CA19-9 Antibody (7E3#)
- 规格:
50ug

| Product name | Anti-sLea/CA19-9 Antibody (7E3#) |
|---|---|
| Catalog No. | HP481207 |
| Host species | Human |
| Specificity | \ |
| Conjugation | Unconjugated |
| Species reactivity | Human |
| Immunogen | \ |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.09% Sodium azide. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | Blocking, ELISA, FCM, IF, IHC |
| Target | CA19-9, CA199, Cancer Antigen 19-9, Cancer Antigen 199, sLea,Sialyl Lewis A |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | \ |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Background | \ |
| Alternative Names | \ |
| Clone ID | \ |
| Species | \ |
| Note | For research use only. |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验人雌三醇 ( E3 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 E3 单抗包被于酶标板上,标准品和样品中的 E3与单抗结合,加入生物素化的抗人 E3 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止液硫酸,在 450nm 处测
(3)同上。两个磷酸化位点都要做,但是可能在不同的病理条件下,侧重点有所不同,这主要体现在你的论文的讨论中。因此建议实验前多做论文研究,查阅一下别人的发表文章,看看与你的病理模型更相关的是哪个位点。 真爱满行囊 非常感谢你 的回复,现在我遇到的问题是我做出来蛋白总量还有216位点是有变化的,9位因为买的是santa curz的抗体,现在死活做不出来,很头痛,我要说明的问题是他的活性降低,我也看到216位点磷酸化水平降低了,可是现在没有第九位的结果(就是没有办法得到第九
Studies of the Ubiquitin Proteasome System
phosphatase inhibitors and 50 µM MG132 (or lactacystin) Anti‐E3 or anti‐substrate antibody Nondenaturing Triton X‐100 wash buffer (see recipe ) Tris‐buffered saline (TBS
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