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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温
- 保质期:
一年
- 英文名:
Fmoc-Thr(tBu)-OH
- 库存:
100
- 供应商:
科淼生物
- CAS号:
71989-35-0
- 规格:
5g
产品中文名称:Fmoc-O-叔丁基-L-苏氨酸
产品英文名称:Fmoc-Thr(tBu)-OH
CAS号:71989-35-0
纯度级别:98%
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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of aggregation plate Materials: Dissecting microscope; Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3); 1 ml syringe with 26 G 1/2 needle of KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);
Metabolic Labeling of Cells with 35S
1) Transfer to a 24 wells plate the desired colonies. 2) Once the cells are attached (at least 8 hours after tripsinizing them) add ~1 ml of DME (met-, cys-). 3) Add 3l of Met 35S, Cys 35S trans label. 4) Incubate at least 5 hours at 370C
The ribonuclease protection assay (RPA)
% acrylamide 9.31 mls of 2% bis acrylamide 7.45 mls of 10x TBE 35.82 g of Urea QS to 74.5 ml with dH2O 450 µl ammonium persulfate (10%) 60 µl TEMED Pour immediately into the gel mold, remove any air bubbles, and add an appropriate comb (e.g
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