Recombinant JEV Capsid protein
C/Core protein Protein (N-GST & C-His Tag), 重组JEVCapsid protein C/Core protein蛋白 (N端GST标签 & C端His标签)- 询价
- abinScience
- France
- VK748012
- 2026年03月03日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期：
1 year
- 英文名：
Recombinant Human PRKRA Protein, N-His
- 库存：
999
- 供应商：
abinScience
- 规格：
100ug
| Expression system | E. coli |
| Accession | Q6UXD5 |
| Protein length | Cys173-Tyr286 |
| Alternative Names | SEZ6L2, PSK, Seizure 6-like protein 2 |
| Form | Lyophilized |
| Predicted molecular weight | 14.61 kDa |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Nature | Recombi\t |
| Species | Homo sapiens (Human) |
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文献和实验Expression and Dimerization of Hepatitis C Virus Core Protein in E. coli
to generate at least 10 viral gene products. Recent reports have indicated that there are extensive interactions between various HCV proteins: the core (capsid) protein can interact with itself (1 ) and with the El envelope protein (2 ); El protein
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
7 mL of 1.15X ligation buffer. 19. Add 400 µL of 20% Triton X-100 (to a final concentration of 1%). Incubate with occasional gentle shaking for 1 h at 37°C. 20. Add 50 µL of T4 DNA ligase. Incubate for 4 h at 16°C
Thrombin Cleavage of GST-Fusion protein
activity keeps the enzyme in aliquots at -80°C. Thrombin (MW 37kDa) ~4500units/mg. One unit cleaves theoretical 100ug protein in 16hr at 22°C in buffer. Another possibility is to use high purity enzyme from Sigma # T-6884: 3-10 U of thrombin can be used
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