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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Lyophilized from a solution in 20 mM Tris-HCl pH 8.0, 100 mM NaCl,1mM EDTA, 4%trehalose,5159% mannitol.
- 保质期:
1 year
- 英文名:
Recombinant PeVYV Putative P3 protein, N-His-SUMO
- 库存:
999
- 供应商:
abinscience
- 规格:
50ug

| Product name | Recombinant PeVYV Putative P3 protein, N-His-SUMO |
|---|---|
| Catalog No. | VK486012 |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | E. coli |
| Accession | A0A7U3TP18 |
| Protein length | Met1-Lys206 |
| Nature | Recombinant |
| Predicted molecular weight | 35.15 kDa |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative Names | Putative P3 protein, PeVYV |
| Species | Pod pepper vein yellows virus (PeVYV) |
| Note | For research use only. |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验Detection of Protein‐Protein Interactions by Coprecipitation
Spring Harbor, N.Y. Knappik, A. and Pluckthun, A. 1994. An improved affinity tag based on the FLAG peptide for the detection and purification of recombinant antibody fragments
如下:几丁质结合蛋白域―intein1―MCS―Intein2―几丁质结合蛋白。这个巧妙的设计有3重便利:N-端,C-端或者N-端和C-端同时融合短的内含肽序列。想做N端融合,你可以将目的基因替换MCS―Intein2―几丁质结合蛋白,如果想做C端融合表达,就可以将目的基因替换MCS前面的几丁质结合蛋白域―intein1―MCS,不用分2个载体;而且做过克隆的人会很有体会:很多时候双酶切载体,由于2个酶切位点都多克隆位点上,没有小片断切下来,所以是否成功双酶切就不好判断,如果用Twin载体就会切下一个片断
前言: NusA融合蛋白表达系统于9年前(1999年)由Davis发明,并在2000年由Novagen首先进行商业化设计和开发,特别推荐用于以NusA作为N端标签时提高在大肠杆菌中难溶的重组表达蛋白的可溶性。NusA融合蛋白表达系统面市至今,已经通过一些高通量表达筛选的评估确认其在可溶性改善方面的有效性。关于NusA融合表达蛋白去除或不去除NusA标签,目的蛋白仍然具有活性的报道也有很多。正是由于NusA标签系统的这种特性,使其成为目前在大肠杆菌表达体系中使用最为广泛的三个融合
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