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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温
- 保质期:
一年
- 英文名:
4-Chlorodiphenyl ether
- 库存:
100
- 供应商:
科淼生物
- CAS号:
7005-72-3
- 规格:
1g
产品中文名称:4-氯二苯醚
产品英文名称:4-Chlorodiphenyl ether
CAS号:7005-72-3
纯度级别:99%
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文献和实验Single Primer ("Semi-Random") P
to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL Cycle as follows: 30"x94°C 20 cycles 0"x94°C 0"x55°C 1'x72° S=9 30 cycles 0"x94°C 0"x40°C 1'x72° S=6 30 cycles 0"x94°C 0"x55°C 1'x72° S=9 Clean up: Add 1 µL ExoI
Single Primer (Semi-Random) PCR
to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL Cycle as follows: 30"x94°C 20 cycles 0"x94°C 0"x55°C 1'x72° S=9 30 cycles 0"x94°C 0"x40°C 1'x72° S=6 30 cycles 0"x94°C 0"x55°C 1'x72° S=9 Clean up: Add 1 µL ExoI nuclease
Assessment of Magnaporthe grisea mating type by spore PCR
by spore PCR are consistent with mating tests on 58 progeny of a cross between strains 4375-R-26 X 4136-4-3. Fungal mycelium can be used instead of spores for PCR, but it may be more difficult to collect mycelium than spores from petri dish culture
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