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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Chlamydia trachomatis Serovar I
- 保质期:
20年
- 供应商:
智立中特(武汉)生物科技有限公司
- 保存条件:
2-8度
- 规格:
甘油
Chlamydia trachomatis Serovar I 拉丁名
(ATCC® VR-880™) 统一编号
Classification Chlamydiacae Deposited As Trachoma serotype i Agent Chlamydia trachomatis Serovar I Strain UW-12/Ur
Biosafety Level 生物安全等级 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen 1 mL per vial
Storage Conditions 保藏方法 -70°C or colder
Comments 注释 Note that activities with high potential for aerosol production require BSL 3 facilities and practices.
Add glassbeads and vortex preparation to disrupt cells.
Infect monolayer with disrupted material.
Centrifuge at 3000 x rpm (750 x g) for 1 hour.
Feed with fresh growth medium containing 1-2 μg/mL cycloheximide.
Incubate at 37°C for 48 hours.
Effect on Host Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa.
Recommended Host McCoy (ATCC® CRL-1696™)
Growth Conditions 生长条件
Temperature 培养温度 : 36°C
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文献和实验Chlamydia trachomatis Serovar I 拉丁名
(ATCC® VR-880™) 统一编号
Classification Chlamydiacae Deposited As Trachoma serotype i Agent Chlamydia trachomatis Serovar I Strain UW-12/Ur
Biosafety Level 生物安全等级 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen 1 mL per vial
Storage Conditions 保藏方法 -70°C or colder
Comments 注释 Note that activities with high potential for aerosol production require BSL 3 facilities and practices.
Add glassbeads and vortex preparation to disrupt cells.
Infect monolayer with disrupted material.
Centrifuge at 3000 x rpm (750 x g) for 1 hour.
Feed with fresh growth medium containing 1-2 μg/mL cycloheximide.
Incubate at 37°C for 48 hours.
Effect on Host Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa.
Recommended Host McCoy (ATCC® CRL-1696™)
Growth Conditions 生长条件
Temperature 培养温度 : 36°C
mahaizhi 各位前辈,请教一下在国内能不能购买到ATCC的原装细胞,有没有哪家公司或者什么机构是ATCC在国内的指定代理。如果有的话,请不吝赐教联系方式。谢谢。 ddh382 我也想知道 veimojie ATCC 在中国的总代理是北京中原公司 比较放心 都是ATCC原装干冰包装发给客户 有兴趣可以去公司主页看看 网址http://www.sinozhongyuan.com/index
海洋微生物的培养基:Marine Agar with Sulfur(ATCC Medium 1922)
Composition per liter:NaCl ……19.45gSulfur ……10.0gMgCl2……8.8gPeptone……5.0gNa2SO3……3.24gCaCl2……1.8gYeast extract ……1.0gKCl……0.55gNaHCO3 ……0.16gFerric citrate……0.1gKBr……0.08gSrCl2……0.03gH3BO3 ……0.02gNa2HPO4 ……8.0mgNa2SiO3……4.0mgNaF……2.4mgNH4NO3 ……
Hairy root clones of Rehmannia glutinosa were established via transformation with Agrobacterium rhizogenes ATCC15834. To optimize the culturing conditions for both root growth and catalpol production, the effects of various combinations
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